NEBNext mRNA Sample Prep Master Mix Set 2

• 产品编号：E6116S      品牌：New England Biolabs       原厂货号：E6116S
• 产品分类：DNA和RNA纯化 > 核酸分析 > 测序试剂和试剂盒 > 测序样本文库制备
• 应用分类：

描述：

Description:
The NEBNext mRNA Library Prep Master Mix Set for 454 contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. Each of these components must pass rigorous quality control standards and are Lot Controlled, both individually and as a set of reagents.

Lot Control:
The lots provided in the NEBNext mRNA Library Prep Master Mix Set for 454 are managed separately and are qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page.

Functionally Validated:
Each set of reagents is functionally validated together through construction and sequencing of a transcriptome library with Roche 454 GS FLX Titanium™ System.

The Master Mix Includes:
The volumes provided are sufficient for preparation of up to 10 reactions (NEB #E6116S) and 50 reactions (NEB #E6116L). (All reagents should be stored at -20°C).

♦ NEBNext RNA Fragmentation Buffer (10X)

♦ NEBNext RNA Fragmentation Stop Solution (10X)

♦ Linear Acrylamide (10 mg/ml)

♦ Random Primers (3 μg/μl)

♦ Murine RNase Inhibitor

♦ NEBNext First Strand Synthesis Reaction Buffer (5X)

♦ NEBNext Second Strand Synthesis Enzyme Mix

♦ NEBNext Second Strand Synthesis Reaction Buffer (10X)

♦ NEBNext End Prep Enzyme Mix

♦ NEBNext End Repair Reaction Buffer (10X)

♦ Quick T4 DNA Ligase

♦ NEBNext Sizing Buffer

♦ TE Buffer

♦ Nuclease-free water

原厂资料：