NEBNext mRNA Sample Prep Master Mix Set 3

• 产品编号：E6114S      品牌：New England Biolabs       原厂货号：E6114S
• 产品分类：DNA和RNA纯化 > 核酸分析 > 测序试剂和试剂盒 > 测序样本文库制备
• 应用分类：

描述：

Description:
The NEBNext mRNA Library Prep Set for SOLiD contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. Each of these components must pass rigorous quality control standards and are Lot Controlled, both individually and as a set of reagents.

Lot Control: The lots provided in the NEBNext mRNA Library Prep Set for SOLiD are managed separately and are qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page.
 

Functionally Validated: Each set of reagents is functionally validated together through construction and sequencing of a transcriptome library on the SOLiD platform (Life Technologies, Inc.).
 

Reagents Supplied

The following reagents are supplied with this product:

♦ NEBNext RNA Fragmentation Buffer (10X)

♦ NEBNext RNA Fragmentation Stop Solution (10X)
♦ Linear Acrylamide (10 mg/ml)
♦ Random Primers (3 μg/μl)

♦ Murine RNase Inhibitor

♦ NEBNext First Strand Synthesis Reaction Buffer (5X)
♦ NEBNext Second Strand Synthesis Enzyme Mix
♦ NEBNext Second Strand Synthesis Reaction Buffer (10X)
♦ NEBNext End Repair Enzyme Mix
♦ NEBNext End Repair Reaction Buffer (10X)
♦ T4 DNA Ligase
♦ NEBNext Quick Ligation Reaction Buffer (5X)
♦ LongAmp® Taq 2X Master Mix

♦ Nuclease-free water
 

原厂资料：