NEBNext mRNA Second Strand Synthesis Module

• 产品编号：E6111L      品牌：New England Biolabs       原厂货号：E6111L
• 产品分类：DNA和RNA纯化 > 核酸分析 > 测序试剂和试剂盒 > 测序专用微珠试剂盒
• 应用分类：

描述：

Description:
 

The NEBNext® Second Strand Synthesis Module has been optimized to generate double stranded cDNA from first strand cDNA. The NEBNext Second Strand Synthesis Module is provided as a master mix to maximize efficiency and convenience in RNA sample preparation workflows.

The dsDNA generated by the NEBNext Second Strand Synthesis Module can be subsequently converted to blunt ended DNA fragments using the NEBNext End Repair Module.

The NEBNext Second Strand Synthesis Module has been validated by sequencing with the Illumina® GAIIx in conjunction with SuperScript® II Reverse Transcriptase, NEBNext First Strand Synthesis Reaction Buffer, Murine RNase Inhibitor, the NEBNext End Repair Enzyme Module, the NEBNext dA-Tailing Module, the NEBNext Quick Ligation Module, Phusion® DNA Polymerase, and Phusion HF Buffer.

The Module Includes:
NEBNext Second Strand Synthesis Enzyme Mix
NEBNext Second Strand Synthesis Reaction Buffer (10X)

The volumes provided are sufficient for preparation of up to 20 reactions (NEB #E6111S) and 100 reactions (NEB #E6111L). (All reagents should be stored at -20°C).

NEBNext Second Strand Synthesis Enzyme Mix
#E6112A: 80 μl
#E6112AA: 400 μl

NEBNext Second Strand Synthesis Enzyme Mix is optimized to conver 10–100 ng of short first strand cDNAs to double-stranded cDNAs with Random Primers and NEBNext Second Strand Synthesis Reaction Buffer.

NEBNext Second Strand Synthesis Enzyme Mix:
6,000 units/ml DNA Polymerase I (E. coli)5,000 units/ml RNase H25,000 units/ml E. coli DNA Ligase

Supplied in: 10 mM Tris-HCl (pH 7.5 @ 25°C), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA and 50% glycerol.

NEBNext Second Strand Synthesis Reaction Buffer
#E6113A: 160 μl
#E6113AA: 800 μl
Concentration: 10X

1X NEBNext Second Strand Synthesis Reaction Buffer:
20 mM Tris-HCl
12 mM (NH4)₂SO₄
5 mM MgCl₂
0.16 mM β-NAD
0.19 mM dNTPs each
pH 7.4 @ 25°C

Applications:

• RNA Sample Preparation
• Second Strand Synthesis of cDNA

Advantages:

• Efficient – Converts 10–100 ng of first strand cDNA into double stranded cDNA
• Convenient – Reactions are provided in master mix format to reduce steps during RNA sample prep workflows
• Automation Friendly

原厂资料：