NEBNext mRNA Library Prep Master Mix Set for Illumina

产品编号：E6110S 品牌：New England Biolabs 原厂货号：E6110S
产品分类：DNA和RNA纯化 > 核酸分析 > 测序试剂和试剂盒 > 测序样本文库制备
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运保温度:

-20°C

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描述：

Description:

The NEBNext® mRNA Library Prep Master Mix Set for Illumina® contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. Each of these components must pass rigorous quality control standards and are Lot Controlled, both individually and as a set of reagents.

Lot Control:
The lots provided in the NEBNext mRNA Library Prep Master Mix Set for Illumina are managed separately and are qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page.

Functionally Validated:
Each set of reagents is functionally validated together through construction and sequencing of a transcriptome library on an Illumina Genome Analyzer IIx.

The Master Mix Set 1 Includes:
The volumes provided are sufficient for preparation of up to 10 reactions (NEB #E6110S) and 50 reactions (NEB #E6110L). (All reagents should be stored at -20°C).

♦ NEBNext RNA Fragmentation Buffer (10X)

♦ NEBNext RNA Fragmentation Stop Solution (10X)

♦ Linear Acrylamide (10 mg/ml)

♦ Random Primers (3 μg/μl)

♦ Murine RNase Inhibitor

♦ NEBNext First Strand Synthesis Reaction Buffer (5X)

♦ NEBNext Second Strand Synthesis Enzyme Mix

♦ NEBNext Second Strand Synthesis Reaction Buffer (10X)

♦ NEBNext End Repair Enzyme Mix

♦ NEBNext End Repair Reaction Buffer (10X)

♦ Klenow Fragment (3´→5´ exo–) (10X)

♦ NEBNext dA-Tailing Reaction Buffer (10X)

♦ Quick T4 DNA Ligase

♦ M-MuLVReverseTranscriptase(RNaseH-)

♦ NEBNext Quick Ligation Reaction Buffer (5X)

♦ Nuclease-free water

♦ Deoxynucleotide Solution Mix (10 mM each dNTP)

♦ Phusion High-Fidelity PCR Master Mix with HF Buffer

♦ NEBNext High-Fidelity 2X PCR Master Mix (SAMPLE)