描述：
Description:
The NEBNext® mRNA Library Prep Reagent Set for Illumina® contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. Each of these components must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.
Lot Control:
The lots provided in the NEBNext mRNA Library Prep Reagent Set for Illuimna are managed separately and are qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page.
Functionally Validated:
Each set of reagents is functionally validated together through construction and sequencing of a transcriptome library on an Illumina Genome Analyzer IIx.
The Reagent Set Includes: The volumes provided are sufficient for preparation of up to 10 reactions (NEB #E6100S) and 50 reactions (NEB #E6100L). (All reagents should be stored at -20°C).
♦ NEBNext® RNA Fragmentation Buffer (10X)
♦ NEBNext® RNA Fragmentation Stop Solution (10X)
♦ Linear Acrylamide (10 mg/ml)
♦ Random Primers (3 μg/μl)
♦ Murine RNase Inhibitor
♦ NEBNext® First Strand Synthesis Reaction Buffer (5X)
♦ NEBNext® Second Strand Synthesis Enzyme Mix
♦ NEBNext® Second Strand Synthesis Reaction Buffer (10X)
♦ Phosphorylation Reaction Buffer (10X)
♦ Deoxynucleotide Solution Mix (10 mM each dNTP)
♦ T4 DNA Polymerase
♦ DNA Polymerase I, Large (Klenow) Fragment
♦ T4 Polynucleotide Kinase
♦ Deoxyadenosine 5´- Triphosphate (dATP) (1.0 mM)
♦ Klenow Fragment (3´→5´ exo–)
♦ NEBuffer® 2 for Klenow Fragment (3´→5´ exo–) (10X)
♦ Quick T4 DNA Ligase
♦ M-MuLV Reverse Transcriptase (RNase H–)
♦ Quick Ligation Reaction Buffer (2X)
♦ Nuclease-free water
♦ Phusion High-Fidelity PCR Master Mix with HF Buffer
♦ NEBNext High-Fidelity 2X PCR Master Mix (SAMPLE)
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