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NEBNext DNA Library Prep Master Mix Set for 454

 
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The NEBNext® DNA Library Prep Master Mix Set for 454™ contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing, and for preparation of single stranded DNA for use in high density hybridization arrays or for genomic subtraction hybridization methods. Each of these components must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents. 

Functional Validation
Each set of reagents is functionally validated together through construction and sequencing of a DNA library on the 454 platform.

Lot Control

The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page

 Reagents Supplied

The following reagents are supplied with this product:

NEBNext End Repair Enzyme Mix

NEBNext End Repair Reaction Buffer

Quick T4 DNA Ligase

NEBNext Quick Ligation Reaction Buffer

Bst DNA Polymerase, Large Fragment

NEBNext Adapter Fill-in Reaction Buffer

Molecular Biology Grade Water

Hydrophilic Streptavidin Magnetic Beads

NEBNext Bead Wash Buffer

NEBNext Bead Binding Buffer


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