Description: The NEBNext DNA Library Prep Set for SOLiD contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing and for preparation of expression libraries. Each of these components must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.
Lot Control:
The lots provided in the NEBNext DNA Library Prep Set for SOLiD are managed separately and are qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page.
Functional Validation:
Each set of reagents is functionally validated together through construction and sequencing of a genomic DNA library on an Applied Biosystems SOLID™ System V3, and by construction of an expression library.
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact OEM@neb.com for further information.
The Reagent Set Includes:
The volumes provided are sufficient for preparation of up to 10 reactions (NEB #E6060S) and 50 reactions (NEB #E6060L). (All reagents should be stored at -20°C):
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