Description: The NEBNext® Quick Ligation Module has been optimized to efficiently ligate DNA adaptors to blunt or dA-Tailed DNA fragments. The NEBNext Quick Ligation Module is provided as a master mix to maximize efficiency and convenience in DNA sample preparation workflows.
The NEBNext Quick Ligation Module has been validated by sequencing with the Illumina Genome Analyzer II in conjunction with the NEBNext End Repair Module, NEBNext dA-Tailing Module and Phusion™ High-Fidelity PCR Master Mix.
The NEBNext Quick Ligation Module has also been validated by sequencing with 454 GS FLX Titanium in conjunction with the NEBNext End Repair Module and the NEBNext Adapter Fill-in and ssDNA Isolation Module.
The Module Includes:
The volumes provided are sufficient for preparation of up to 20 reactions (NEB #E6056S) and 100 reactions (NEB #E6056L). (All reagents should be stored at -20°C).
Quick T4 DNA Ligase
#E6057A: 0.1 ml
#E6057AA: 0.5 ml
NEBNext Quick Ligation Reaction Buffer (5X)
#E6058A: 0.2 ml
#E6058AA: 1.0 ml
1X NEBNext Quick Ligation Reaction Buffer:
66 mM Tris-HCl
10 mM MgCl2
1 mM dithiothreitol
1 mM ATP
6% Polyethylene glycol (PEG 6000)
pH 7.6 @ 25°C
Applications:
DNA Sample Preparation
Ligation of DNA adaptors to blunt or dA-Tailed DNA
Ligation of blunt or dA-Tailed DNA and cloning vectors
Advantages:
Efficient – Ligates blunt DNA or dA-Tailed DNA
Convenient – Reactions are provided in master mix format to reduce steps during DNA sample prep workflows
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