Taq PCR Kit

产品编号：E5000S 品牌：New England Biolabs 原厂货号：E5000S
产品分类：PCR/RT-PCR/qRT-PCR > PCR > 常规PCR
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包装：

运保温度:

-20°C

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描述：

♦ Robust and reliable reactions

♦ Tolerates a wide range of templates

♦ Incorporates dUTP, dITP and fluorescently-labeled nucleotides

♦ Exceptional value in terms of cost per unit

Description:
The Polymerase Chain Reaction (PCR) is a powerful and sensitive technique for DNA amplification (1). PCR amplifies specific DNA sequences exponentially by using multiple cycles of a three-step process. First, the double-stranded DNA template is denatured at a high temperature. Sequence-specific primers are then annealed to sites flanking the target sequence. A thermostable DNA polymerase, such as Taq DNA Polymerase (2–6), then extends the annealed primers, thereby doubling the amount of the original DNA sequence. This newly synthesized product then becomes an additional template for subsequent cycles of amplification. These three steps are repeated for 20 to 30 cycles, resulting in a 10⁵ –10⁹ fold increase in target DNA concentration.

Kit Components:
Taq DNA Polymerase with Standard Taq Buffer (5,000 units/ml)
Deoxynucleotide Solution Mix (200 μl)
Magnesium Chloride (MgCl₂) Solution (1.5 ml)
Quick-Load® 2-Log DNA Ladder(0.1-10.0 kb) (200 μl)
Standard Taq (Mg-free) Reaction Buffer Pack (1.5 ml)
Standard Taq Reaction Buffer Pack (1.5 ml)