Luciferase 1000 Assay System

• 产品编号：E4550      品牌：promega       原厂货号：E4550
• 产品分类：蛋白质检测与分析 > 酶检测和活性分析 > 荧光素酶检测
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其它组分：

描述：

Luciferase Assay System ( 萤光素酶检测系统) 可以非常灵敏快速地定量检测萤火虫萤光素酶。可在至少8 个数量级的酶浓度范围内得到线性结果。专利的试剂配方可在最佳条件下测量少于10^-20摩尔的萤光素酶。其灵敏度通常都高于氯霉素转乙酰基酶 (CAT) 检测法100 倍。Luciferase Assay Reagent 产生的发光可稳定至少一分钟，因此可与单管的发光仪以及带有自动注射器的多孔板发光仪器配合使用检测萤火虫萤光素酶活性。

萤光素酶检测系统是非均质检测系统；即含有萤光素酶的细胞必须在加入试剂前被裂解。Glo Lysis Buffer( 光亮裂解缓冲液，Cat.#E2661)，Cell Culture Lysis Reagent( 细胞培养物裂解试剂，Cat.#E1531)，Passive Lysis Buffer( 被动裂解缓冲液，Cat.# E1941) 和Reporter Lysis Buffer( 报告基因裂解缓冲液，Cat.# E3971) 可与萤光素酶检测系统一起定量检测哺乳动物细胞中的报告基因。萤光素酶检测系统也可用于植物和细菌细胞的定量检测，但只有细胞培养物裂解试剂适合这些应用。应用报告基因裂解缓冲液可对同一细胞裂解液进行萤火虫萤光素酶、CAT 和β- 半乳糖苷酶等多项检测。有些试剂盒包括裂解缓冲液，而有些试剂盒需单独购买裂解缓冲液。

原厂资料：

The Luciferase Assay System is substantially improved over conventional assay methods in both sensitivity and simplicity. Light is produced by converting the chemical energy of luciferin oxidation through an electron transition, forming the product molecule oxyluciferin. Firefly luciferase, a monomeric 61kDa protein, catalyzes luciferin oxidation using ATP-Mg²⁺ as a cosubstrate. In the conventional assay for luciferase, a flash of light is generated that decays rapidly after the enzyme and substrates are combined. The Luciferase Assay System incorporates coenzyme A (CoA) for improved kinetics, allowing greater enzymatic turnover and resulting in increased light intensity that is nearly constant for at least 1 minute. The Luciferase Assay System yields linear results over at least eight orders of magnitude. Less than 10^-20 moles of luciferase have been detected under optimal conditions. Generally, 100-fold greater sensitivity can be achieved over the chloramphenicol acetyltransferase (CAT) assay.
The Luciferase Assay System was developed for reporter quantitation in mammalian cells. The Luciferase Assay System (Cat.# E1500), provided with Cell Culture Lysis Reagent (CCLR), also can be used for reporter quantitation in plant and bacterial cells; however, the Luciferase Assay System with Reporter Lysis Buffer (Cat.# E4030) is not suitable for these applications.
The Luciferase Reporter 1000 Assay System (Cat.# E4550) was designed to meet the needs of users who perform a large number of assays, particularly in 96-well plates. The system contains sufficient reagents to perform 1,000 luciferase assays (100µl per assay). For users working with transformed cells, a cell lysis buffer will be needed for sample preparation prior to luciferase measurement. The lysis buffer must be purchased separately.

注意事项：

储存于-20℃,Reporter Lysis Buffer (Cat.# E3971) 可储存于室温。