ENZCHEK PEPTIDASE/PROTEASE 1 KIT

• 产品编号：E33758      品牌：invitrogen停用       原厂货号：E33758
• 产品分类：蛋白质检测与分析 > 酶检测和活性分析 > 蛋白酶/肽酶检测
• 应用分类：

描述：

The EnzChek® Peptidase/Protease Assay Kit enables simple and accurate quantification of a wide range of protease activities within 60 minutes.

See our complete line of Fluorescence Microplate assays.

• Simple mix-incubate-read format
• Excitation and emission wavelengths (502/528 nm) well suited for fluorescein (FITC) filter settings
• Easily adapted to a variety of proteases and reaction conditions

The EnzChek® peptidase/protease substrate included in the kit has been labeled with a fluorophore and a quencher moiety separated by a peptide. Upon cleavage by protease(s), the fluorophore separates from the quencher and, upon excitation, the fluorophore emits fluorescence signal. The magnitude of the resultant signal is proportional to the degree of substrate cleavage, resulting in an ability to quantify the amount of enzyme activity. The assay is performed in a simple mix–incubate–read format, can be completed in 60 minutes or less, and is easily adapted to accommodate diverse pH requirements.

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