HeLaScribe? Nuclear Extract in vitro Transcription Grade

产品编号：E3091 品牌：promega 原厂货号：E3091
产品分类：克隆 > 体外转录 > 体外转录
应用分类：

包装：

40 reactions

运保温度:

-70℃

到货周期：

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其它组分：

HeLaScribe® Nuclear Extract Transcription Grade【子货号：E309A，包装：2 x 20 reactions，，运保温度：-70℃】

描述：

HeLaScribe^®Nuclear Extract，in vitro Transcription Grade(HeLaScribe^® 核抽提物，体外转录级别) 由HeLa 细胞核提取，是真核基因的一种体外转录系统。

原厂资料：

The most well characterized cell-free system for in vitro transcription of eukaryotic genes is derived from HeLa cell nuclei. HeLa nuclear extracts can support accurate transcription initiation by RNA polymerase II and exhibit both basal and regulated patterns of transcription. These nuclear extracts also are a source of a variety of transcription factors, DNA-binding proteins and the enzymatic machinery involved in RNA processing.
The HeLaScribe® Nuclear Extract included in this system is prepared by a modification of the method of Dignam et al. (Nucl. Acids Res. 11, 1475). The system includes all of the necessary components for in vitro transcription as well as a positive control template (CMV immediate early promoter DNA).