Glo Lysis Buffer, 1X

• 产品编号：E2661      品牌：promega       原厂货号：E2661
• 产品分类：蛋白质检测与分析 > 酶检测和活性分析 > 荧光素酶检测
• 应用分类：蛋白质研究 > 报告基因检测 > 荧光素酶报告基因检测

描述：

Glo Lysis Buffer(GLB), 1X, 是专利配方，可快速裂解(5 分钟内)哺乳动物细胞，而不需刮除或反复冻融。与Bright-Glo™, Steady-Glo®, ONE-Glo™ 和 Renilla-Glo™ Luciferase Assay Reagents，以及用于分析萤火虫萤光素酶的表达的萤光素酶检测试剂完全兼容。在GLB存在和不存在条件下，这些试剂的半衰期都相同，分别为Steady-Glo^® 试剂 >5 小时，Bright-Glo^™ 试剂>24 分钟。

原厂资料：

Glo Lysis Buffer (GLB), 1X, is a proprietary formulation developed to promote rapid lysis (within 5 minutes) of cultured mammalian cells without scraping or performing freeze-thaw cycles. It is fully compatible with Bright-Glo™, Steady-Glo®, ONE-Glo™ and Renilla-Glo® Luciferase Assay Reagents and the Luciferase Assay Reagent for analysis of firefly luciferase expression. The half-life of these reagents remains the same with or without use of GLB, >5 hours for Steady-Glo® Reagent and >24 minutes for Bright-Glo™ Reagent.

Features - Benefits

• Convenient: No need for cell scraping or freeze-thaw cycles.
• Fast: Cell lysis within 5 minutes.
• Versatile: Use with Bright-Glo™, Steady-Glo®, ONE-Glo™ and Renilla-Glo® Luciferase Assay Reagents to provide nonhomogeneous assay formats or with other reporter applications.
• Robust: Firefly luciferase enzyme in Glo Lysis Buffer is stable at room temperature for at least 48 hours.

注意事项：

储存于4℃。要长期保存，需冻存于-20℃或-70℃。