pGL3-Basic Vector

• 产品编号：E1751      品牌：promega       原厂货号：E1751
• 产品分类：克隆 > 克隆载体和克隆试剂盒 > 载体 > 报告基因载体
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The pGL3 Luciferase Reporter Vectors provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These may becis- or trans-acting factors. The backbone of the pGL2 Luciferase Reporter Vectors was redesigned for the pGL3 Vectors for increased expression, with a modified coding region for firefly (Photinus pyralis) luciferase that has been optimized for monitoring transcriptional activity in transfected eukaryotic cells. The assay of this genetic reporter is rapid, sensitive and quantitative. In addition, the Luciferase Reporter Vectors contain numerous features aiding in the structural characterization of the putative regulatory sequences under investigation.

For the most advanced reporter vectors and widest selection of features, please see the pGL4 Luciferase Reporter Vectors.

Features - Benefits

• Easy to Use: NcoI site located at 5´ end of luc+ gene allows creation of fusions with reporter gene using a unique NcoI site.
• Flexible: Placement of SmaI site in the MCS allows blunt-ended inserts to be ligated into the MCS and restricted on either side by other restriction endonucleases.
• Versatile: XbaI site just downstream of luc+ gene facilitates insertions into the 3´ untranslated region of mRNA or subcloning of the luciferase gene.