Luciferase Assay System

• 产品编号：E1500      品牌：promega       原厂货号：E1500
• 产品分类：蛋白质检测与分析 > 酶检测和活性分析 > 荧光素酶检测
• 应用分类：蛋白质研究 > 报告基因检测 > 荧光素酶报告基因检测

其它组分：

描述：

Luciferase Assay System ( 萤光素酶检测系统) 可以非常灵敏快速地定量检测萤火虫萤光素酶。可在至少8 个数量级的酶浓度范围内得到线性结果。专利的试剂配方可在最佳条件下测量少于10^-20摩尔的萤光素酶。其灵敏度通常都高于氯霉素转乙酰基酶 (CAT) 检测法100 倍。Luciferase Assay Reagent 产生的发光可稳定至少一分钟，因此可与单管的发光仪以及带有自动注射器的多孔板发光仪器配合使用检测萤火虫萤光素酶活性。

萤光素酶检测系统是非均质检测系统，即含有萤光素酶的细胞必须在加入试剂前被裂解。Glo Lysis Buffer( 光亮裂解缓冲液，Cat.#E2661)，Cell Culture Lysis Reagent( 细胞培养物裂解试剂，Cat.#E1531)，Passive Lysis Buffer( 被动裂解缓冲液，Cat.# E1941) 和Reporter Lysis Buffer( 报告基因裂解缓冲液，Cat.# E3971) 可与萤光素酶检测系统一起定量检测哺乳动物细胞中的报告基因。萤光素酶检测系统也可用于植物和细菌细胞的定量检测，但只有细胞培养物裂解试剂适合这些应用。应用报告基因裂解缓冲液可对同一细胞裂解液进行萤火虫萤光素酶、CAT 和β- 半乳糖苷酶等多项检测。

有些试剂盒包括裂解缓冲液，而有些试剂盒需单独购买裂解缓冲液。

原厂资料：

The Luciferase Assay System is an extremely sensitive and rapid reagent for quantitation of firefly luciferase. Linear results are seen over at least eight orders of magnitude of enzyme concentration, and patented technology incorporated in the formulation has allowed for less than 10^–20 moles of luciferase to be measured under optimal conditions. Generally, 100-fold greater sensitivity can be achieved over the chloramphenicol acetyltransferase (CAT) assay. The Luciferase Assay Reagent generates light that is nearly constant for at least 1 minute and so is compatible with measuring firefly luciferase in a single-tube luminometer or in a multiwell plate luminometer with an auto-injector.

The Luciferase Assay System is a nonhomogeneous assay system; the cells containing the luciferase must be lysed before reagent addition. Glo Lysis Buffer (Cat.# E2661), Cell Culture Lysis Reagent (Cat.# E1531), Passive Lysis Buffer (Cat.# E1941) and Reporter Lysis Buffer (Cat.# E3971) may be used with the Luciferase Assay System for reporter quantitation in mammalian cells. The Luciferase Assay System may also be used for quantitation in plant and bacterial cells, but only Cell Culture Lysis Reagent is suitable for these applications. Reporter Lysis Buffer allows for firefly luciferase, CAT and β-galactosidase assays to be performed from the same cell extract. In some kits the lysis buffer is included, and in others it must be purchased separately (see Component Listing link above).

Features - Benefits

• Linear: Eight or more orders of magnitude of enzyme concentration.
• Sensitive: To 10^–20 moles of luciferase.
• Fast: Perform cell lysis, sample preparation and assays in as little as 5 minutes.
• Convenient: Reporter Lysis Buffer allows luciferase, CAT and β-galactosidase assays to be performed from the same cell extract.
• Simple Assay Procedure: Eliminates the need for autoinjection devices and rapid mixing protocols when using single-tube luminometers.
• Versatile: Luminometer preferred, but not required; adaptable to scintillation counters.
• Safe: Non-radioactive.
• Superior: High performance compared to competitors' luciferase assays.

Applications

For more information, see the Protocols & Applications Guide.

References

1. Wood, K.V. (1991) In: Bioluminescence & Chemiluminescence: Current Status, eds. P. Stanley and L. Kricka, John Wiley & Sons, Ltd., Chichester, 543.
2. Seliger, H.H. and McElroy, W.D. (1960) Arch. Biochem. Biophys. 88, 136.
3. Wood, K.V. et al. (1984) Biochem. Biophys. Res. Comm. 124, 592–6.
4. de Wet, J.R. et al. (1985) Proc. Natl. Acad. Sci. USA 82, 7870–3.
5. Alam, J. and Cook, J.L. (1990) Anal. Biochem. 188, 245–54.
6. Schenborn, E. and Goiffon, V. (1993) Promega Notes 41, 11–3.
7. Jones, D.P. et al. (1995) Promega Notes 54, 20–5.

注意事项：

储存于-20℃