Quick Blunting and Quick Ligation Kits

• 产品编号：E0542L      品牌：New England Biolabs       原厂货号：E0542L
• 产品分类：克隆 > 克隆载体和克隆试剂盒 > 依赖DNA连接酶的克隆试剂盒
• 应用分类：

描述：

Description:
NEB offers the Quick Blunting™ and Quick Ligation™ Kits together as a bundle at a value price.

The Quick Blunting™ Kit is used to convert DNA with incompatible 5´or 3´overhangs to 5´phosphorylated, blunt-ended DNA for efficient blunt-end ligation into DNA cloning vectors. DNA is blunted using T4 DNA polymerase (NEB #M0203) which has both 3´→ 5´ exonuclease activity and 5´→ 3´ polymerase activity. T4 Polynucleotide Kinase (NEB #M0201) is included in the enzyme mix for phosphorylation of the 5´ends of blunt-ended DNA for subsequent ligation into a cloning vector. This kit is optimized for blunting up to 5 µg of DNA in a single reaction.

The Quick Ligation™ Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature (25°C).

原厂资料：

注意事项：

Please note storage conditions for individual products.