DC055 is a dual colour reagent containing antibodies which recognise murine CD115 and CD11b cell surface antigens.
Antibodies produced by clone 604B5 2E11 recognise the murine CD115 cell surface antigen, also known as the M-CSF receptor and c-fms.CD115 is expressed by cells of the monocytic lineage and by progenitor cells.
Antibodies produced by clone M1/70.15 recognise the murine CD11b cell surface antigen (also known as the alpha M integrin chain or MAC-1), a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Flow Cytometry
Neat - 1/5
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents.
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