DC053 is a dual colour reagent containing antibodies which recognise murine Ly-6C and CD31 cell surface antigens.
Antibodies produced by clone ERMP20 recognise murine Ly-6C, a 14kD differentiation antigen, which is expressed on macrophage/dendritic cell precursors in mid-stage development (late CFU-M, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B- and T-lymphocytes.
Antibodies produced by clone ERMP12 recognise murine CD31, a 140kD cell surface glycoprotein that is expressed at high levels on endothelial cells, platelets, and most leukocyte subpopulations.CD31 is also expressed on a major population of macrophage / dendritic cell precursors in the bone marrow.
Studies show that clone ER-MP12 can be used in conjunction with clone ER-MP20 in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Flow Cytometry
Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents.
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