Dimethyl sulfoxide, or DMSO, is a highly polar organic solvent that is miscible with water. Many compounds that are not directly soluble in water can be dissolved with DMSO to create a concentrated stock solution that can be further diluted to the desired final concentration in aqueous buffer or media at the time of use.
Molecular Probes® DMSO is suitable for preparing stock solutions of many popular fluorescent reagents including:
• Fluorescent calcium indicators (e.g. Fluo-3, AM, Fura-2, AM)
• Fluorescent probes for labeling mitochondria (e.g. rhodamine 123, JC-1, MitoTracker® Green or Red)
• Fluorescent probes for cell tracking or tracing (e.g. CFSE, calcein, AM, CellTracker™ Green CMFDA)
• Fluorescent probes for labeling membranes (e.g. DiI or DiO)
• Fluorescent reactive oxygen species (ROS) indicators (e.g. DCFDA, CM-DCFDA, dihydroethidium or Amplex Red® reagent)
• Amine-reactive fluorescent labeling reagents (e.g. Alexa Fluor® 488 NHS ester or fluorescein isothiocyanate (FITC))
• Thiol-reactive fluorescent labeling reagents (e.g. Alexa Fluor® 488 or fluorescein maleimide)
Molecular Probes® DMSO is provided in convenient single-use aliquots to ensure fresh, high quality DMSO every time you prepare a concentrated DMSO stock solution. Please refer to the individual online product manuals for specific instructions on the recommended DMSO stock and working solution concentration for your product.
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