Thermo Scientific Accell Red Non-targeting Control siRNA provides qualitative assessment of Accell siRNA uptake as determined by fluorescent microscopy to evaluate cytoplamic localization of the dye-labeled Accell siRNA or FACS analysis to determine general uptake. Non-targeting siRNA reagents are designed to aid in the interpretation of experiments with Accell siRNA reagents as highly reliable controls for assessment of baseline cellular responses.
Highlights
Designed, modified, and microarray-confirmed to have minimal targeting of known genes in human, mouse, and rat cells
Modified with patent-pending Accell modification pattern to enable uptake by difficult-to-transfect cells
Accell modifications also provide stability against nuclease-mediated degradation
Accell Red is labeled with DY-547 (Cy3 analog). Absorbance/ Emission Max is 557/570 nm; a Cy3, Rhodamine or PE filter can be used
Experimental considerations
Accell siRNA works at a higher concentration than conventional siRNA; recommended 1 µM working concentration
Delivery may be inhibited by the presence of BSA in serum. Optimization studies with serum-free media formulations (Accell Delivery Media) or < 2.5% serum in standard media is recommended
Full-serum media can be added back after 48 hours of incubation. Optimal mRNA silencing is typically achieved by 72 hours or up to 96 hours for protein knockdown
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