Thermo Scientific Accell Green Non-targeting Control siRNA provides qualitative assessment of Accell siRNA uptake as determined by fluorescent microscopy to evaluate cytoplamic localization of the dye-labeled Accell siRNA or FACS analysis to determine general uptake. Non-targeting siRNA reagents are designed to aid in the interpretation of experiments with Accell siRNA reagents as highly reliable controls for assessment of baseline cellular responses.
Highlights
• Designed, modified, and microarray-confirmed to have minimal targeting of known genes in human, mouse, and rat cells
• Modified with patent-pending Accell modification pattern to enable uptake by difficult-to-transfect cells
• Accell modifications also provide stability against nuclease-mediated degradation
• Accell Green is labeled with 6-FAM. Absorbance/Emission Max is 494/520 nm; a FITC filter can be used
Experimental considerations
• Accell siRNA works at a higher concentration than conventional siRNA; recommended 1 µM working concentration
• Delivery may be inhibited by the presence of BSA in serum. Optimization studies with serum-free media formulations (Accell Delivery Media) or < 2.5% serum in standard media is recommended
• Full-serum media can be added back after 48 hours of incubation. Optimal mRNA silencing is typically achieved by 72 hours or up to 96 hours for protein knockdown
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