The Thermo Scientific Accell Non-Targeting Control Pool is designed to aid in the interpretation of experiments with Accell siRNA reagents as highly reliable controls for assessment of baseline cellular responses. Each of the four component siRNAs is designed with no homology to known human, mouse, or rat genes. RNAi experiments should include a non-targeting siRNA or pool as a control for non-sequence-specific effects. Changes in mRNA or protein levels in cells treated with these controls reflect a non-specific baseline cellular response that can be compared to the levels in cells treated with target-specific siRNA.
Highlights
• Pool of four siRNAs designed to have minimal targeting of known genes in human, mouse, and rat cells
• Modified with patent-pending Accell modification pattern to enable uptake by difficult-to-transfect cells
• Accell modifications also provide stability against nuclease-mediated degradation
Experimental considerations
• Accell siRNA works at a higher concentration than conventional siRNA; recommended 1 µM working concentration
• Delivery may be inhibited by the presence of BSA in serum. Optimization studies with serum-free media formulations (Accell Delivery Media) or < 2.5% serum in standard media is recommended
• Full-serum media can be added back after 48 hours of incubation. Optimal mRNA silencing is typically achieved by 72 hours, or up to 96 hours for protein knockdown
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