Thermo Scientific Accell Non-Targeting Control siRNA 1, 2, 3, and 4 are designed to aid in the interpretation of experiments with Accell siRNA reagents as highly reliable controls for assessment of baseline cellular responses. RNAi experiments should include a non-targeting siRNA or pool as a control for non-sequence-specific effects. Changes in mRNA or protein levels in cells treated with these controls reflect a non-specific baseline cellular response that can be compared to the levels in cells treated with target-specific siRNA.
Highlights
• Designed, modified, and microarray-confirmed to have minimal targeting of known genes in human, mouse, and rat cells
• Modified with patent-pending Accell modification pattern to enable uptake by difficult-to-transfect cells
• Accell modifications also provide stability against nuclease-mediated degradation
Experimental considerations
• Accell siRNA works at a higher concentration than conventional siRNA; recommended 1 µM working concentration
• Delivery may be inhibited by the presence of BSA in serum. Optimization studies with serum-free media formulations (Accell Delivery Media) or < 2.5% serum in standard media is recommended
• Full-serum media can be added back after 48 hours of incubation. Optimal mRNA silencing is typically achieved by 72 hours or up to 96 hours for protein knockdown
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