收藏本站
热门搜索: A3500  E1910  A1360  25200056  dmem  

siGLO Lamin A/C siRNA (Human/Mouse/Rat)

 
包装: 20 nmol
运保温度: -20 C
到货周期: 登录后查看
标准价: ¥客户可见
会员价: ¥客户可见
积分: 客户可见
 
登录之后可加入购物车购买,请您 
对比 收藏

运费与支付说明:

1.含干冰类产品有运费;

2. 必须现金付款或有信用额度的会员才可以直接发货,否则需要等待现金付款信息。

描述:

通过转染常规的荧光标记siRNA,然后进行荧光观测评价转染效率与siRNA的RISC结合和基因沉默并不相关。这是由多种因素所决定的,包括siRNA的荧光标签断裂和荧光的迅速悴灭。同时,当用于共转染实验时,有研究表明,如果靶基因siRNA没有经过优化设计,则荧光标记的siRNA将和实验组siRNA竞争性结合RISC(见下图),从而降低实验中siRNA的沉默效果。siGLOTM siRNA是一种全新的、稳定的、荧光标记的对照产品,适合于人和小鼠的对照产品。
                                   
Figure . Silencing efficiency of siCONTROL™ Cyclophilin B siRNA in the presence of increasing amounts of siGLO™ RISC-Free™ siRNA. HeLa cells were co-transfected with a constant concentration of siRNA (100 nM) comprised of increasing ratios of Cyclophilin B siRNA:RISC-Free™ (or Non-Targeting) siRNA. For comparison, a parallel set of samples included cells transfected with cyclophilin B siRNA alone. After 24 hours, cyclophilin B mRNA levels were measured using a branched DNA assay (Genospectra).When unmodified, nontargeting siRNA is included in the transfection mix, the level of targeted gene silencing is reduced in a dose-dependent fashion suggesting competition for the silencing machinery. In contrast, when RISC-Free™ siRNA is a part of the transfection mix, no effect on the silencing efficiency (no competition) is observed.
 
产品特点:
  • 可靠的转染效率评价。
  • 荧光信号强烈、时间持久,可达7天甚至更久。
  • 荧光强度和转染效率、靶基因沉默效果呈正相关。
  • 与实验组siRNA共转染,不影响实验组siRNA的基因沉默效果

 

阳性对照 siGLO Lamin A/C siRNA (HumanMouseRat)
产品描述:
siGLO Lamin A/C siRNA试剂的靶基因为Lamin A/C,Lamin基因介导灯丝型蛋白构成主要的核纤层组成。Lamin A/C高表达于大部分的人、小鼠和大鼠细胞,Lamin A/C基因mRNA的敲低并不影响细胞活性。此阳性对照产品经过化学修饰,与传统的荧光标记siRNA相比,增强了siRNA的稳定性和荧光强度。每一种siRNA均特异性靶向人、小鼠和大鼠的Lamin基因。

 
产品特点:
  • 靶基因序列号:NM_005572, NM_170707, NM_170708 (人);NM_019390 (小鼠) ;NM_001002016 (大鼠)。
  • 值得信赖的转染效率评价工具。
  • 独特的化学修饰增强了荧光强度和持久性(可达7天甚至更长时间)。
  • 荧光与转染效率和靶基因沉默效果正相关。
  •                                 
    Figure. Fluorescence microscope images of HeLa cells transfected with stable and unmodified forms of cyclophilin B siRNA. In addition to the fluorescent label, siGLO™ siRNAs contain modifications that increase their stability in cell culture. The added stability is evident in these side-byside comparisons of HeLa cell cultures transfected with siGLO™ or unmodified forms of Cyclophilin B siRNA (dye-labeled for fluorescence detection). Note that siCONTROL™ Cyclophilin B siRNA does not usually contain a fluorescent label.


相关图片

说明书

参考文献

本产品可用于的实验

购此产品的人还购买了
最近浏览产品

京ICP备15036693号-2    京公网安备11010802025653    版权所有:北京逸优科技有限公司       0.04

 纠 错