Figure . Silencing efficiency of siCONTROL™ Cyclophilin B siRNA in the presence of increasing amounts of siGLO™ RISC-Free™ siRNA. HeLa cells were co-transfected with a constant concentration of siRNA (100 nM) comprised of increasing ratios of Cyclophilin B siRNA:RISC-Free™ (or Non-Targeting) siRNA. For comparison, a parallel set of samples included cells transfected with cyclophilin B siRNA alone. After 24 hours, cyclophilin B mRNA levels were measured using a branched DNA assay (Genospectra).When unmodified, nontargeting siRNA is included in the transfection mix, the level of targeted gene silencing is reduced in a dose-dependent fashion suggesting competition for the silencing machinery. In contrast, when RISC-Free™ siRNA is a part of the transfection mix, no effect on the silencing efficiency (no competition) is observed.
产品特点：
可靠的转染效率评价。
荧光信号强烈、时间持久，可达7天甚至更久。
荧光强度和转染效率、靶基因沉默效果呈正相关。
与实验组siRNA共转染，不影响实验组siRNA的基因沉默效果
阳性对照 siGLO Lamin A/C siRNA (Human，Mouse，Rat)
产品描述：
siGLO Lamin A/C siRNA试剂的靶基因为Lamin A/C，Lamin基因介导灯丝型蛋白构成主要的核纤层组成。Lamin A/C高表达于大部分的人、小鼠和大鼠细胞，Lamin A/C基因mRNA的敲低并不影响细胞活性。此阳性对照产品经过化学修饰，与传统的荧光标记siRNA相比，增强了siRNA的稳定性和荧光强度。每一种siRNA均特异性靶向人、小鼠和大鼠的Lamin基因。
Figure. Fluorescence microscope images of HeLa cells transfected with stable and unmodified forms of cyclophilin B siRNA. In addition to the fluorescent label, siGLO™ siRNAs contain modifications that increase their stability in cell culture. The added stability is evident in these side-byside comparisons of HeLa cell cultures transfected with siGLO™ or unmodified forms of Cyclophilin B siRNA (dye-labeled for fluorescence detection). Note that siCONTROL™ Cyclophilin B siRNA does not usually contain a fluorescent label.
原厂资料：
Thermo Scientific siGLO Lamin A/C Control siRNA is a validated positive control, guaranteed to silence Lamin A/C in human cells. This control siRNA is chemically modified to significantly extend siRNA stability and fluorescence compared to conventional siRNAs similarly labeled with fluorophores.
Lamins are intermediate filament-type proteins, which form major components of the nuclear lamina. Lamins A and C have close sequence homology and are derived by differential splicing and alternative polyadenylation from one gene. This gene is abundantly expressed in most cells, and because it is non-essential, knockdown of the corresponding mRNA does not affect cell viability. Targets accession numbers: NM_005572, NM_170707, NM_170708 (Human), NM_019390 (Mouse), and NM_001002016 (Rat).
Highlights
• Labeled with DY-547 (Cy3 analog) – Absorbance/Emission Max = 557/570 nm
• Significantly extended intensity of the fluorescence signal (7 days or longer)
• Positive correlation of cytoplasmic fluorescence with transfection efficiency
To request a larger quantity or in vivo processing of this control product, please email Technical Support, or call 1-800-235-9880. International customers, please call 303-604-9499 or your local Sales Representative.
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