siGLO Cyclophilin B Control siRNA (Human/Mouse/Rat)

产品编号：D-001610-01-20 品牌：Dharmacon 原厂货号：D-001610-01-20
产品分类：DNA和RNA纯化 > RNA干扰 > RNA干扰对照 > RNAi阳性对照
应用分类：

包装：

20 nmol

运保温度:

-20 C

到货周期：

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描述：

通过转染常规的荧光标记siRNA，然后进行荧光观测评价转染效率与siRNA的RISC结合和基因沉默并不相关。这是由多种因素所决定的，包括siRNA的荧光标签断裂和荧光的迅速悴灭。同时，当用于共转染实验时，有研究表明，如果靶基因siRNA没有经过优化设计，则荧光标记的siRNA将和实验组siRNA竞争性结合RISC（见下图），从而降低实验中siRNA的沉默效果。siGLO^TM siRNA是一种全新的、稳定的、荧光标记的对照产品，适合于人和小鼠的对照产品。

Figure . Silencing efficiency of siCONTROL™ Cyclophilin B siRNA in the presence of increasing amounts of siGLO™ RISC-Free™ siRNA. HeLa cells were co-transfected with a constant concentration of siRNA (100 nM) comprised of increasing ratios of Cyclophilin B siRNA:RISC-Free™ (or Non-Targeting) siRNA. For comparison, a parallel set of samples included cells transfected with cyclophilin B siRNA alone. After 24 hours, cyclophilin B mRNA levels were measured using a branched DNA assay (Genospectra).When unmodified, nontargeting siRNA is included in the transfection mix, the level of targeted gene silencing is reduced in a dose-dependent fashion suggesting competition for the silencing machinery. In contrast, when RISC-Free™ siRNA is a part of the transfection mix, no effect on the silencing efficiency (no competition) is observed.

产品特点：

可靠的转染效率评价。
荧光信号强烈、时间持久，可达7天甚至更久。
荧光强度和转染效率、靶基因沉默效果呈正相关。
与实验组siRNA共转染，不影响实验组siRNA的基因沉默效果

阳性对照 siGLO Cyclophilin B siRNA (Human/Mouse/Rat)

产品描述：
siGLO Cyclophilin B siRNA试剂的靶基因为Cyclophilin B（peptidylprolyl isomerase B 也叫PPIB），其荧光强度和沉默活性正相关。Cyclophilin B基因的siRNA是一种常见的阳性沉默对照，CyclophilinB通常定位于内质网，和内分泌途径相关。Cyclophilin B高表达于大部分的人、小鼠和大鼠细胞，Cyclophilin B基因mRNA的敲低并不影响细胞活性。此阳性对照产品经过化学修饰，与传统的荧光标记siRNA相比，增强了siRNA的稳定性和荧光强度。同一siRAN产品可同时作为人、小鼠和大鼠的阳性对照产品。

产品特点：

靶基因序列号：NM_000942 (人) , NM_011149 (小鼠) 和 NM_022536 (大鼠) 。
值得信赖的转染效率评价工具。
独特的化学修饰增强了荧光强度和持久性（可达7天甚至更长时间）。
荧光与转染效率和靶基因沉默效果正相关。

原厂资料：

Thermo Scientific siGLO Cyclophilin B Control siRNA is a validated positive control, guaranteed to silence cyclophilin B in human, mouse, or rat cells. This control siRNA is chemically modified to significantly extend siRNA stability and fluorescence compared to conventional siRNAs similarly labeled with fluorophores.

Also known as peptidylprolyl isomerase B (PPIB), cyclophilin B is associated with the secretory pathway. This gene is abundantly expressed in most cells, and because it is non-essential, knockdown of the corresponding mRNA does not affect cell viability.

Highlights
• Targets accession numbers NM_000942 (Human), NM_011149 (Mouse), or NM_022536 (Rat)
• Labeled with DY-547
• Absorbance/Emission Max is 557/570 nm
• A Cy3, Rhodamine or PE filter can be used
• Significantly extended intensity of the fluorescence signal (7 days or longer)
• Positive correlation of fluorescence with transfection efficiency and target gene silencing
To request a larger quantity or in vivo processing of this control product, please email Technical Support, or call 1-800-235-9880. International customers, please call 303-604-9499 or your local Sales Representative.