siGLO RISC-Free Control siRNA

产品编号：D-001600-01-05 品牌：Dharmacon 原厂货号：D-001600-01-05
产品分类：DNA和RNA纯化 > RNA干扰 > RNA干扰对照 > RNAi阴性对照
应用分类：

包装：

5 nmol

运保温度:

-20 C

到货周期：

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描述：

通过转染常规的荧光标记siRNA，然后进行荧光观测评价转染效率与siRNA的RISC结合和基因沉默并不相关。这是由多种因素所决定的，包括siRNA的荧光标签断裂和荧光的迅速悴灭。同时，当用于共转染实验时，有研究表明，如果靶基因siRNA没有经过优化设计，则荧光标记的siRNA将和实验组siRNA竞争性结合RISC（见下图），从而降低实验中siRNA的沉默效果。siGLO^TM siRNA是一种全新的、稳定的、荧光标记的对照产品，适合于人和小鼠的对照产品。

Figure . Silencing efficiency of siCONTROL™ Cyclophilin B siRNA in the presence of increasing amounts of siGLO™ RISC-Free™ siRNA. HeLa cells were co-transfected with a constant concentration of siRNA (100 nM) comprised of increasing ratios of Cyclophilin B siRNA:RISC-Free™ (or Non-Targeting) siRNA. For comparison, a parallel set of samples included cells transfected with cyclophilin B siRNA alone. After 24 hours, cyclophilin B mRNA levels were measured using a branched DNA assay (Genospectra).When unmodified, nontargeting siRNA is included in the transfection mix, the level of targeted gene silencing is reduced in a dose-dependent fashion suggesting competition for the silencing machinery. In contrast, when RISC-Free™ siRNA is a part of the transfection mix, no effect on the silencing efficiency (no competition) is observed.

产品特点：

可靠的转染效率评价。
荧光信号强烈、时间持久，可达7天甚至更久。
荧光强度和转染效率、靶基因沉默效果呈正相关。
与实验组siRNA共转染，不影响实验组siRNA的基因沉默效果

转染指示剂 siGLO RISC-Free siRNA

产品特点：
稳定、荧光标记、无靶点的对照产品。
标记DY-547（Absorbance: 557, Emission Max: 570 nm）。
不和RISC结合，可有效区分转染siRNA引起的细胞应激效应。
可以和有功能siRNA共同转染而不影响基因沉默效率。

原厂资料：

Thermo Scientific siGLO RISC-Free Control siRNA is chemically modified to impair processing and uptake by RISC, allowing researchers to isolate cellular effects related to siRNA transfection. As transfection reagents typically rely on charged interaction with the nucleic acid payload, transfection controls using siGLO RISC-Free are recommended over the use of transfection reagent alone.

Highlights
• Stable, fluorescent, negative RNAi control
• Labeled with DY-547
• Absorbance/Emission Max is 557/570 nm
• A Cy3, Rhodamine or PE filter can be used
• Not processed by RISC - useful to isolate cellular effects related only to siRNA transfection
• Enables co-transfection with functional siRNA without interfering with target gene silencing
To request a larger quantity or in vivo processing of this control product, please email Technical Support, or call 1-800-235-9880. International customers, please call 303-604-9499 or your local Sales Representative.