siGENOME Non-Targeting siRNA #2

• 产品编号：D-001210-02-05      品牌：Dharmacon       原厂货号：D-001210-02-05
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNA干扰对照 > RNAi阴性对照
• 应用分类：

描述：

siGENOME siRNA阳性对照：

产品特点：

对人，小鼠和大鼠高丰度表达的看家基因进行有效持久的沉默效果。

应用：

通过检测人、大鼠和小鼠目的基因的沉默效率来确定siRNA相应的转染效率。
为使用Individual siRNA和SMARTpool试剂进行体外研究实验提供了配套的工具。

siGENOME siRNA阴性对照：

产品特点：
无靶标的siRNAs产品或Pool产品设计时未针对人、大鼠和小鼠细胞的任何特定基因。
无靶标siRNAs已经通过微阵列验证在人源细胞中几乎无其靶向标的。
有RISC-Free siRNA供选择，对该产品进行化学修饰可降低其导入效率。
应用：

在人、大鼠和小鼠中确定siSTABLE individual siRNA的沉默特异性。
可从非特异性效应中区别出序列特异性沉默效应。

原厂资料：

Thermo Scientific siGENOME Non-Targeting Control siRNAs are highly functional, chemically synthesized, and mostly unmodified negative control siRNA duplexes for experiments in human, mouse, and rat cells. siGENOME Non-Targeting Controls were designed to have a minimum of 4 mismatches to all human, mouse and rat genes, and confirmed to have minimal targeting by genomewide microarray analysis. Changes in mRNA or protein levels in cells treated with these controls reflect a baseline cellular response that can be compared to the levels in cells treated with target-specific siRNA.

Highlights
• BLAST analysis confirms at least 4 mismatches with all known human, mouse, and rat genes
• Screened by microarray analysis

Experimental Considerations
•siGENOME Non-targeting siRNA #1 reduces EGFR mRNA (NM_005228) in an assay- and cell-specific manner by ~50%. Originally reported by customers, this finding was not observed by initial microarray validation, but has been subsequently verified by internal scientists. Recommended only if you have successfully used this control in past experiments
•siGENOME Non-Targeting siRNA #2 and #4 target firefly luciferase mRNA (U47296). These siRNAs can be used either as a non-targeting siRNA in human, mouse or rat cell lines or as a positive silencing control in systems using firefly luciferase as a reporter gene (pGL3 cloning vector, Promega).
•siGENOME Non-Targeting siRNA #5 carries the ON-TARGET modification pattern to prevent sense strand uptake.