siGENOME Non-Targeting siRNA Pool #2

• 产品编号：D-001206-14-50      品牌：Dharmacon       原厂货号：D-001206-14-50
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNA干扰对照 > RNAi阴性对照
• 应用分类：

描述：

siGENOME siRNA阳性对照：

产品特点：

对人，小鼠和大鼠高丰度表达的看家基因进行有效持久的沉默效果。

应用：

通过检测人、大鼠和小鼠目的基因的沉默效率来确定siRNA相应的转染效率。
为使用Individual siRNA和SMARTpool试剂进行体外研究实验提供了配套的工具。

siGENOME siRNA阴性对照：

产品特点：
无靶标的siRNAs产品或Pool产品设计时未针对人、大鼠和小鼠细胞的任何特定基因。
无靶标siRNAs已经通过微阵列验证在人源细胞中几乎无其靶向标的。
有RISC-Free siRNA供选择，对该产品进行化学修饰可降低其导入效率。
应用：

在人、大鼠和小鼠中确定siSTABLE individual siRNA的沉默特异性。
可从非特异性效应中区别出序列特异性沉默效应。

原厂资料：

Thermo Scientific siGENOME Non-Targeting siRNA Control Pools are highly functional, chemically synthesized, and mostly unmodified negative control siRNA duplexes for experiments in human mouse and rat cells. siGENOME Non-Targeting Control Pools are each a pool of four siRNAs, allowing the effective concentration of each siRNA to be reduced, and thus further minimize off-target effects. siGENOME Non-Targeting Control siRNAs were designed to have a minimum of 4 mismatches to all human, mouse and rat genes, and confirmed to have minimal targeting by genomewide microarray analysis.

Changes in mRNA or protein levels in cells treated with these controls reflect a baseline cellular response that can be compared to the levels in cells treated with target-specific siRNA.

Highlights
• BLAST analysis confirms at least 4 mismatches with all known human, mouse, and rat genes
• Characterized by microarray analysis to have minimal off-targeting
• Minimized off-target effects due to pooling strategy; effective concentration of each siRNA is reduced

Experimental Considerations

siGENOME Non-Targeting Pool #1 is comprised of Non-Targeting siRNAs #1 through 4

siGENOME Non-Targeting Pool #2 is comprised of Non-Targeting siRNAs #2 through 5
• siGENOME Non-targeting siRNA #1 reduces EGFR mRNA (NM_005228) in an assay- and cell-specific manner by ~50%, this off-target is decreased in the context of the Control Pool #1, but for this reason Non-Targeting Control pool #2 is recommended.
• siGENOME Non-Targeting siRNA #2 and #4 target firefly luciferase mRNA (U47296).