In general, antibodies to collagen in human sera are highly specifi c to heterologous collagen such as bovine or chick type I and/or type II collagen and cross-react to other species and types of collagen including human collagen, regardless of the disease. For example, serum antibodies even from healthy normal controls react to various species and types of collagen. To determine the human serum antibodies with a significant diversity in the antigen specificity, Chondrex provides various species of type I and type II collagen-coated strips as well as uncoated wells (see table above).This ELISA kit contains enough materials to run two plates on two separate occasions (see assay procedure) and may be used for monkey sera as well as human sera.
原厂资料：
注意事项：
NOTES BEFORE USING ASSAY
1. It is recommended that the standard and samples be run in duplicate.
2. Partially used reagents may be kept at –20°C.
3. Crystals may form in the 20X wash buffer when stored at cold temperatures. If crystals have formed, it is necessary to warm the wash
buffer by placing the bottle in warm water until crystals have dissolved completely.
4. Measure exact volume of buffers using a serological pipette prior to diluting. Extra buffer is provided
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