ELISA Grade Mouse Type I Collagen, 0.5 mg/ml x 1ml

• 产品编号：Cd-1006      品牌：Chondrex       原厂货号：1006
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞外基质蛋白 > 胶原
• 应用分类：

原厂资料：

Chondrex provides highly purified, polymeric-free ELISA grade collagen. Various species and types of collagen can be used as an antigen in
ELISA for studying antibody specificity and cross-reactivity (Table 1). Since collagen is a rigid fibrilar protein with unique physical and chemical
properties and differs from other globular proteins, special attention for handling this protein is required. For example, immunoglobulins in
human and animal sera bind to polymeric and fibrilar collagen non-specifically, and create significantly high false-positive reaction in ELISA.
Similarly, a secondary antibody in ELISA also binds to fibrilar collagen non-specifically and creates high background values. Furthermore, these
forms of collagen affect the assay sensitivity significantly due to the formation of excess collagen layer on the surfaces of ELISA plate.
ELISA grade collagen is supplied as a 100X concentrated solution (0.5 mg/mL). Each vial contains enough collagen to coat ten, 96-well plates.
A 10X Collagen Dilution Buffer (catalog # 9003) is also included per order of ELISA grade collagen to prevent fibril formation.

注意事项：

The binding efficiency of collagen on ELISA plates depends upon the type of plastic and pre-treatment of the surface. Therefore, we
recommend using a high binding plate such as Immulon 2 HB. Mouse monoclonal anti-type II collagen antibody (catalog # 7005),
which cross-reacts to all species of type II collagen listed in Table 1, can be used as a positive control or to study the binding efficiency
of individual species of type II collagen on ELISA plates.