Oligomycin

• 产品编号：CST-9996L      品牌：CST       原厂货号：9996L
• 产品分类：细胞,细胞培养与转染试剂 > 抗生素和抗真菌剂 > 其它
• 应用分类：

描述：

寡霉素在二甲亚砜(DMSO），丙酮和乙醇中可溶。在线粒体氧化磷酸化过程中，电子传递链将氢离子从线粒体基质泵到内外膜中层。这样就形成了一个跨线粒体内膜的质子梯度。质子梯度可以驱动线粒体内膜上的F0-F1 ATP合酶，将ADP转化成ATP。寡霉素，是一种淀粉酶产色链丝菌产生的抗生素，它可以通过结合到ATP合酶上的F0组分来抑制其活性，并阻断氧化磷酸化过程(1-3

1. Kagawa, Y. and Racker, E. (1966) J Biol Chem 241, 2461-6.
2. Kagawa, Y. and Racker, E. (1966) J Biol Chem 241, 2467-74.
3. Fessenden, J.M. and Racker, E. (1966) J Biol Chem 241, 2483-9.

原厂资料：

Directions for Use

Oligomycin is soluble in DMSO, acetone or ethanol.

Background

During oxidative phosphorylation in mitochondria, the electron transport system pumps H+ from the matrix into the intermembrane compartment. This process generates a H+ gradient across the mitochondrial inner membrane. This H+ gradient is used to drive mitochondrial FoF1 ATPase to convert ADP to ATP. Oligomycin, an antibiotic from Streptomyces diastatochromogenes, blocks oxidative phosphorylation by binding to the Fo part of the ATPase to inhibit its activity (1-3).

1. Kagawa, Y. and Racker, E. (1966) J Biol Chem 241, 2461-6.
2. Kagawa, Y. and Racker, E. (1966) J Biol Chem 241, 2467-74.
3. Fessenden, J.M. and Racker, E. (1966) J Biol Chem 241, 2483-9.

注意事项：

Storage: Store lyophilized or in solution at -20°C, desiccated.
Protect from light. In lyophilized form, the chemical is stable for
24 months. Once in solution, use within 3 months to prevent loss
of potency. Aliquot to avoid multiple freeze/thaw cycles.