Ionomycin Calcium Salt

• 产品编号：CST-9995S      品牌：CST       原厂货号：9995S
• 产品分类：细胞,细胞培养与转染试剂 > 抗生素和抗真菌剂 > 其它
• 应用分类：

描述：

onomycin钙盐以2.5mg粉末提供。储存在-20℃。使用前，将粉末溶解于1.1ml DMSO中产生3mM原液。对于工作浓度为1uM-3uM，将原液按1:3000到1:1000稀释。使用需要的浓度和时间处理细胞。Ionomycin在粉剂状态比溶液中更稳定；因此推荐在使用前再将其溶解。储存在-20℃或更低温度。离子霉素（Ionomycin）是一个强有力的选择性钙离子载体（1,2）。该分子可以以运动型钙离子载体发挥作用，通过直接刺激存储调节的阳离子通过生物膜进入细胞而加强Ca2+内流（3）。在微摩尔水平，ionomycin可以激活钙/钙调素依赖性蛋白激酶和磷酸酶来刺激基因的表达（4）。在人类T细胞中，ionomycin诱导磷酸肌醇水解，并激活蛋白激酶C介导的T细胞活化（5）。Ionomycin处理人类B细胞可诱导激活钙依赖的核酸内切酶并导致细胞凋亡（6）。Ionomycin处理小牛主动脉内皮细胞（BAEC）可诱导快速的eNOS Thr495去磷酸化，介导eNOS活化（7）。

1. Liu, C. and Hermann, T.E. (1978) J Biol Chem 253, 5892-4.
2. Kauffman, R.F. et al. (1980) J Biol Chem 255, 2735-9.
3. Morgan, A.J. and Jacob, R. (1994) Biochem J 300 ( Pt 3), 665-72.
4. Lobo, F.M. et al. (1999) J Immunol 162, 2057-63.
5. Chatila, T. et al. (1989) J Immunol 143, 1283-9.
6. Aagaard-Tillery, K.M. and Jelinek, D.F. (1995) J Immunol 155, 3297-307.
7. Lin, M.I. et al. (2003) J Biol Chem 278, 44719-26.

原厂资料：

Directions for Use

Ionomycin, calcium salt is supplied as 2.5 mg powder. Store at or below -20ºC. Before use, dissolve powder in 1.1 ml DMSO to make a 3 mM ionomycin stock solution. For working concentrations of 1 μM-3 μM, dilute DMSO stock 1:3000 to 1:1000. Treat cells with the desired concentration for intended time. Ionomycin is more stable as a powder than in solution; it is therefore recommended to dissolve as close as possible prior to use. Store solution at or below -20ºC.

Background

Ionomycin is a potent and selective calcium ionophore agent (1,2). The molecule acts as a motile Ca2+ carrier and enhances Ca2+ influx by direct stimulation of store-regulated cation entry across biological membranes (3). At the micromolar level, ionomycin can activate Ca2+/Calmodulin dependent kinase and phosphatase to stimulate gene expression (4). In human T cells, ionomycin induces hydrolysis of phosphoinositides and activates PKC to mediate T cell activation (5). Ionomycin treatment of human B cells induces the activation of calcium-dependent endonuclease and results in apoptosis (6). Ionomycin treatment of bovine aortic endothelial cells (BAECs) induces rapid dephosphorylation of eNOS at Thr495 and mediates eNOS activation (7).

1. Liu, C. and Hermann, T.E. (1978) J Biol Chem 253, 5892-4.
2. Kauffman, R.F. et al. (1980) J Biol Chem 255, 2735-9.
3. Morgan, A.J. and Jacob, R. (1994) Biochem J 300 ( Pt 3), 665-72.
4. Lobo, F.M. et al. (1999) J Immunol 162, 2057-63.
5. Chatila, T. et al. (1989) J Immunol 143, 1283-9.
6. Aagaard-Tillery, K.M. and Jelinek, D.F. (1995) J Immunol 155, 3297-307.
7. Lin, M.I. et al. (2003) J Biol Chem 278, 44719-26.

注意事项：

This product is for in vitro research use only and is not intended for use in humans or animals.

Storage: Store lyophilized or in solution at -20°C, desiccated.
Protect from light. In lyophilized form, the chemical is stable for
24 months. Once in solution, use within 3 months to prevent loss
of potency. Aliquot to avoid multiple freeze/thaw cycles.