His-Tag (27E8) Mouse mAb (HRP Conjugate)

• 产品编号：CST-9991S      品牌：CST       原厂货号：9991S
• 产品分类：抗体 > 一抗 > 酶标记一抗 > 辣根过氧化物酶（HRP）
• 应用分类：

描述：

His-Tag (27E8) Mouse mAb (HRP Conjugate)能检测包含6xHis附加表位的重组蛋白。此抗体能识别转染细胞中融合到靶蛋白氨基端或羧基端的6xHis标签。单克隆抗体通过用6xHis合成肽免疫动物制备。此抗体通过它的胺基团结合到辣根过氧化物酶（HRP）的糖类基团。HRP结合的抗体与unconjugated His-Tag (27E8) Mouse mAb #2366有相同的种交叉反应。附加表位对于用免疫印迹、免疫沉淀和免疫染色技术来进行的蛋白质标记和检测很有用。因为它们很小，不大可能会影响被标记蛋白的生化特性。很多质粒都包含一个DNA编码的氨基端标签，该标签由6个组氨酸(6xHis)残基组成，后面有扩展的多克隆位点。表达的重组蛋白上的6xHis标签能有效地结合到Ni2+亲和树脂上，通过单步色谱法纯化（1）。与其他蛋白质标记系统一样（2），此多组氨酸标签常常能被蛋白酶（如凝血酶和肠激酶）在位点上识别并裂解来分离相关蛋白（1）。

1. Kroll, D.J. et al. (1993) DNA Cell Biol. 12, 441-453.
2. di Guan, C. et al. (1988) Gene 67, 21-30.

原厂资料：

Specificity / Sensitivity

His-Tag (27E8) Mouse mAb (HRP Conjugate) detects recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the 6xHis-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a 6xHis synthetic peptide.

Description

This Cell Signaling Technology® antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated His-Tag (27E8) Mouse mAb #2366.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.A variety of plasmids contain DNA that encodes an amino-terminal tag consisting of six histidine (6xHis) residues followed by an extended multiple cloning site. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography (1).As is the case with other protein tag systems (2), this polyhistidine tag can often be cleaved at sites recognized by proteases such as thrombin and enterokinases to isolate the protein of interest (1).

1. Kroll, D.J. et al. (1993) DNA Cell Biol. 12, 441-453.
2. di Guan, C. et al. (1988) Gene 67, 21-30.

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM
sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50%
glycerol. Store at –20°C. Do not aliquot the antibody.