Cyclosporin A

• 产品编号：CST-9973S      品牌：CST       原厂货号：9973S
• 产品分类：生化试剂 > 生物试剂 > 生物活性小分子 > 抑制剂
• 应用分类：

描述：

本产品的包装规格是100mg粉末。-20ºC保存。Cyclosporin A粉末能够溶解在乙醇（或者DMSO）中而配制成母液，母液也需要在-20ºC保存。工作液的浓度和处理时长可根据不同的实验目的而变化，通常工作液的浓度约为100 nM。钙离子依赖的蛋白磷酸酶--钙调磷酸酶能够对活化T细胞核因子(NFAT)的转录调节因子去磷酸化，其对活化T细胞核因子的细胞核定位和活化也有着重要的作用(1,2)。钙调磷酸酶是cyclosporin A (CsA) (3) 和 FK-506 （又叫他克莫司和藤霉素）(4)两个免疫抑制剂的重要靶点，cyclosporin A (CsA)和 FK-506 能够抑制抗原和分裂素对T细胞的激活，这两个药物分别与亲免疫因子cyclophilin和FKBP-12结合形成亲免疫因子-药物复合物并与钙调磷酸酶结合从而阻止其与底物的结合(5)。在很多研究模型中FK-506的效力比CsA高100多倍(6-8)。

1. Schulz, R.A. and Yutzey, K.E. (2004) Dev Biol 266, 1-16.
2. Rusnak, F. and Mertz, P. (2000) Physiol Rev 80, 1483-521.
3. Borel, J.F. et al. (1976) Agents Actions 6, 468-75.
4. Kino, T. et al. (1987) J Antibiot (Tokyo) 40, 1249-55.
5. Liu, J. et al. (1991) Cell 66, 807-15.
6. Henderson, D.J. et al. (1991) Immunology 73, 316-21.
7. Tocci, M.J. et al. (1989) J Immunol 143, 718-26.
8. Yoshimura, N. et al. (1989) Transplantation 47, 351-6.

原厂资料：

Directions for Use

Cyclosporin A is supplied as a 100 mg powder. Store at -20ºC. Cyclosporin A is soluble in ethanol (also DMSO) and stock solutions should be stored at -20ºC. Working concentrations and length of treatment can vary depending on the desired effect but it is typically used at around 100 nM.

Background

The calcium dependent protein phosphatase calcineurin is responsible for the de-phosphorylation of the transcriptional regulator nuclear factor of activated T cells (NFAT) and is essential for NFAT’s nuclear translocation and activation (1,2). Calcineurin is a target of two common immunosuppressants, cyclosporin A (CsA) (3) and FK-506 (also know as tacrolimus and fugimycin) (4), both of which can inhibit antigen and mitogen triggered T cell activation. These drugs interact with the immunophilins cyclophilin and FKBP-12, respectively, and the immunophilin-drug complex binds to calcineurin to inhibit substrate binding (5). FK-506 can be up to 100-fold more potent than CsA in various models (6-8).

1. Schulz, R.A. and Yutzey, K.E. (2004) Dev Biol 266, 1-16.
2. Rusnak, F. and Mertz, P. (2000) Physiol Rev 80, 1483-521.
3. Borel, J.F. et al. (1976) Agents Actions 6, 468-75.
4. Kino, T. et al. (1987) J Antibiot (Tokyo) 40, 1249-55.
5. Liu, J. et al. (1991) Cell 66, 807-15.
6. Henderson, D.J. et al. (1991) Immunology 73, 316-21.
7. Tocci, M.J. et al. (1989) J Immunol 143, 718-26.
8. Yoshimura, N. et al. (1989) Transplantation 47, 351-6.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Store lyophilized or in solution at -20°C, desiccated.
Protect from light. In lyophilized form, the chemical is stable for
24 months. Once in solution, use within 3 months to prevent loss
of potency. Aliquot to avoid multiple freeze/thaw cycles.