Brefeldin A

• 产品编号：CST-9972S      品牌：CST       原厂货号：9972S
• 产品分类：细胞,细胞培养与转染试剂 > 抗生素和抗真菌剂 > 其它
• 应用分类：

描述：

Brefeldin A被提供5 mg粉末。存储-20ºC。Brefeldin A可溶于DMSO(乙醇和甲醇)，并且存储液（10 mg/ml）应该存储在-20ºC。工作浓度和处理时间长度依据期望的效应而不同。从内质网(ER)转运到高尔基体(Golgi)的抑制作用在至少100 ng/ml时被观察，而凋亡在10 μg/ml的延长处理时被观察。 Brefeldin A (BFA)是一个真菌代谢物，其被证明可逆地干扰从内质网向高尔基体转运(1,2)。当起初分离的作为一个抗生素(3)，并且有一个广泛的抗生素活性，它主要作为研究蛋白转运的生物研究工具。BFA处理后导致在内质网上一个快速的蛋白堆积，并且高尔基体会被破坏。BFA的处理也能抑制蛋白分泌(4)和延长的给药时间能诱导凋亡(5)。BFA的主要目标出现是ADP-ribosylation factor (ARF)，该蛋白复制对外壳蛋白到高尔基膜的关联(6,7)。

1. Klausner, R.D. et al. (1992) J Cell Biol 116, 1071-80.
2. Pelham, H.R. (1991) Cell 67, 449-51.
3. Tamura, G. et al. (1968) J Antibiot (Tokyo) 21, 160-1.
4. Misumi, Y. et al. (1986) J Biol Chem 261, 11398-403.
5. Shao, R.G. et al. (1996) Exp Cell Res 227, 190-6.
6. Helms, J.B. and Rothman, J.E. (1992) Nature 360, 352-4.
7. Randazzo, P.A. et al. (1993) J Biol Chem 268, 9555-63.

原厂资料：

Directions for Use

Brefeldin A is supplied as a 5 mg powder. Store at -20ºC. Brefeldin A is soluble in DMSO (also ethanol and methanol) and stock solutions (typically 10 mg/ml) should be stored at -20ºC. Working concentrations and length of treatment can vary depending on desired effect. Inhibiton of ER to Golgi trafficking was observed as low as 100 ng/ml and apoptosis was observed with prolonged treatment at 10 μg/ml. 

Background

Brefeldin A (BFA) is a fungal metabolite demonstrated to reversibly interfere with anterograde transport from the endoplasmic reticulum to the Golgi apparatus (1,2). While initially isolated as an antibiotic (3), and does have a wide range of antibiotic activity, it is primarily used as a biological research tool for studying protein transport. Treatment leads to a rapid accumulation of proteins within the ER and collapse of the Golgi stacks. Treatment with BFA can also inhibit protein secretion (4) and prolonged exposure can induce apoptosis (5). The main target of BFA appears to be ADP-ribosylation factor (ARF), which is responsible for association of coat protein to the Golgi membrane (6,7).

1. Klausner, R.D. et al. (1992) J Cell Biol 116, 1071-80.
2. Pelham, H.R. (1991) Cell 67, 449-51.
3. Tamura, G. et al. (1968) J Antibiot (Tokyo) 21, 160-1.
4. Misumi, Y. et al. (1986) J Biol Chem 261, 11398-403.
5. Shao, R.G. et al. (1996) Exp Cell Res 227, 190-6.
6. Helms, J.B. and Rothman, J.E. (1992) Nature 360, 352-4.
7. Randazzo, P.A. et al. (1993) J Biol Chem 268, 9555-63.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Store lyophilized or in solution at -20°C, desiccated.
In lyophilized form, the chemical is stable for 24 months. Once in
solution, use within 3 months to prevent loss of potency. Aliquot
to avoid multiple freeze/thaw cycles.