Staurosporine

• 产品编号：CST-9953S      品牌：CST       原厂货号：9953S
• 产品分类：生化试剂 > 生物试剂 > 生物活性小分子 > 抑制剂
• 应用分类：

描述：

用法说明：用DMSO或者甲醇溶解冻干生物碱。1mM溶液溶解于0.536mL。Staurosporine（星孢菌素）是链霉菌肉汤培养液中提取的生物碱。它是强有效的细胞渗透的蛋白酶C抑制剂，IC50是0.7nM。高浓度（1-20nM）时，staurosporine 也可以抑制其它酶的活性，例如PKA、PKG、CAMKII和球蛋白轻链酶（1）。浓度为50-100nM时，它是作为神经营养因子的激动剂，促进神经母细胞瘤、嗜铬细胞瘤和脑原发神经元神经轴突外生。浓度为0.2-1uM时，staurosporine 诱导细胞凋亡。（2，3）

1. Ruegg, U.T. and Burgess, G.M. (1989) Trends Pharmacol. Sci. 10, 218-220.
2. Couldwell , W. T. et al. (1994) FEBS Lett. 345, 43-46.
3. Yue, T. L. et al. (1998) J. Mol. Cell. Cardiol. 30, 495-507.

原厂资料：

Directions for Use

Dissolve the lyophilized alkaloid in DMSO or Methanol. For a 1 mM solution, dissolve in 0.536 mL.

Background

Staurosporine is an alkaloid isolated from the culture broth of Streptomyces staurosporesa. It is a potent, cell permeable protein kinase C inhibitor with an IC50 of 0.7 nM. At higher concentration (1-20 nM), staurosporine also inhibits other kinases such as PKA, PKG, CAMKII and Myosin light chain kinase (MLCK) (1). At 50-100 nM, it is a functional neurotrophin agonist, promoting neurite outgrowth in neuroblastoma, pheochromocytoma and brain primary neuronal cultures. At 0.2- 1 μM, staurosporine induces cell apoptosis (2,3).

1. Ruegg, U.T. and Burgess, G.M. (1989) Trends Pharmacol. Sci. 10, 218-220.
2. Couldwell , W. T. et al. (1994) FEBS Lett. 345, 43-46.
3. Yue, T. L. et al. (1998) J. Mol. Cell. Cardiol. 30, 495-507.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Store lyophilized or in solution at -20ºC, desiccated.
Protect from light. In lyophilized form, the chemical is stable for
24 months. Once in solution, use within 3 months to prevent loss
of potency. Aliquot to avoid multiple freeze/thaw cycles.