Wortmannin

• 产品编号：CST-9951S      品牌：CST       原厂货号：9951S
• 产品分类：生化试剂 > 生物试剂 > 生物活性小分子 > 抑制剂
• 应用分类：

描述：

储存浓度为2 mM，将1 mg化合物溶于1.16 ml的DMSO中。用于细胞实验，CST建议wortmannin的浓度控制在0.2 µm 和 1 µm 之间，在刺激之前一小时加入，直到刺激结束。进一步信息，请参阅的MSDS。Wortmannin是一个非常有效的，特异的和直接的PI3K激酶抑制剂，最初由真菌获得（1,2）。它的抑制作用是不可逆转的，非竞争性的。Wortmannin不抑制PI4K激酶，蛋白激酶C或酪氨酸蛋白激酶（3）

1. Nakanishi, S. et al. (1992) J. Biol. Chem. 267, 2157-2163.
2. Arcaro, A. and Wymann, M.P. (1993) Biochem. J. 296, 297-301.
3. Powis, G. et al. (1994) Cancer Res. 54, 5241-5248.

原厂资料：

Directions for Use

For 2 mM stock, dissolve 1 mg in 1.16 ml DMSO. For experiments with cultured cells, CST recommends treating with wortmannin at concentrations between 0.2 µm and 1 µm, for one hour prior to, and for the duration of, the stimulation. See MSDS for further information.

Background

Wortmannin is a very potent, specific and direct inhibitor of PI3 kinase, originally derived from fungus (1,2). The inhibition is irreversible and noncompetitive. Wortmannin does not inhibit PI4 kinase, protein kinase C or protein tyrosine kinase (3).

1. Nakanishi, S. et al. (1992) J. Biol. Chem. 267, 2157-2163.
2. Arcaro, A. and Wymann, M.P. (1993) Biochem. J. 296, 297-301.
3. Powis, G. et al. (1994) Cancer Res. 54, 5241-5248.

注意事项：

Storage: Store lyophilized or in solution at -20°C, desiccated.
Protect from light. In lyophilized form, the chemical is
stable for 24 months. Once in solution, use within 3 months
to prevent loss of potency. Aliquot to avoid multiple freeze/
thaw cycles.