Trichostatin A (TSA)

• 产品编号：CST-9950S      品牌：CST       原厂货号：9950S
• 产品分类：细胞,细胞培养与转染试剂 > 抗生素和抗真菌剂 > 其它
• 应用分类：

描述：

警惕：该化合物需要进行极稀溶液稀释且只能被用于生物研究过程中。不能用于其他意图，任何其他使用都可能产生重大危害。化合物不能以粉剂或者气溶胶以及其他形式存在与实验室中。避光-20ºC保存。 Trichostatin A为低压冻干粉末。加入8264µL 无水乙醇使之成为4mM（10 ,000X）保存母液。使用时稀释至终浓度为400 nM，处理12-18小时。Trichostatin A 是一种从链霉菌属中分离得到的抗真菌的抗生素，可以抑制哺乳动物细胞组蛋白脱乙酰基酶（1，2）。在纳摩尔浓度下，Trichostatin A可以特异性和可逆性的加速组蛋白乙酰化（2，3）。研究表明Trichostatin A还可以抑制哺乳动物细胞周期中的G1期和G2期，可以用于抗肿瘤药物的研究中（2-5）。

1. Tsuji, N. et al. (1976) J. Antibiot. 29, 1-6.
2. Yoshida, M. et al. (1990) J. Biol. Chem. 265, 17174-17179.
3. Kijima, M. et al. (1993) J. Biol. Chem. 268, 22429-22435.
4. Ailenberg, M. and Silverman, M. (2002) Biochem. Biophys. Res. Commun. 298, 110-115.
5. Maecker, H. et al. (2002) Cancer Cell 2, 139-148.

原厂资料：

Directions for Use

Precautions: 
 
 This compound is only sold for use in extremely dilute solutions for biological research. No other use is intended and any other use involves substantial hazards. This compound should never be handled in powder or aerosol form or in any other form susceptible to uncontrolled release in the laboratory, even in very small quantities. 
 
 Store at -20ºC in the dark. Trichostatin A is supplied as a lyophilized powder. For a 4mM stock (10.000X), reconstitute in 826 µL ethanol. Treat cells with 400 nM trichostatin A for 12-18 hours.

Background

Trichostatin A is an antifungal antibiotic derived from Streptomyces that inhibits mammalian histone deacetylases (1,2). When used at nanomolar concentrations, trichostatin A causes the accumulation of acetylated histones in a quite specific and reversible manner (2,3). Trichostatin A has also been shown to inhibit both G1- and G2-phases of the mammalian cell cycle and has been tested for use as a potential anticancer agent (2-5).

1. Tsuji, N. et al. (1976) J. Antibiot. 29, 1-6.
2. Yoshida, M. et al. (1990) J. Biol. Chem. 265, 17174-17179.
3. Kijima, M. et al. (1993) J. Biol. Chem. 268, 22429-22435.
4. Ailenberg, M. and Silverman, M. (2002) Biochem. Biophys. Res. Commun. 298, 110-115.
5. Maecker, H. et al. (2002) Cancer Cell 2, 139-148.

注意事项：

Storage: Store lyophilized or in solution at -20°C, desiccated.
Protect from light. In lyophilized form, the chemical is stable for
24 months. Once in solution, use within 3 months to prevent loss
of potency. Aliquot to avoid multiple freeze/thaw cycles