AICAR

• 产品编号：CST-9944S      品牌：Cell Signaling Technology       原厂货号：9944S
• 产品分类：生化试剂 > 生物试剂 > 生物活性小分子 > 激活剂
• 应用分类：

描述：

AICAR产品浓度为250 mM。对于0.5 mM的工作浓度，将原液按1:500稀释，对于2 mM的工作浓度，按1:125稀释。细胞应于预期浓度下处理5-60min。需要注意的是：AICAR是清澈透明的呈黄棕色的溶液，不应有沉淀。如果发现有沉淀，将盖子拧紧后，用体温加热小瓶，直到沉淀溶解。必要时，可以边加热边摇动溶液，促进其溶解。此产品仅用于体外实验，不适用于动物和人体研究。此产品同样不适用于治疗和诊断。AICAR以250 mM浓度的水溶液提供。使用前应充分解冻融。对于0.5 mM的工作浓度，将原液按1:500稀释，对于2 mM的工作浓度，按1:125稀释。细胞于预期浓度下处理5-60min。AICAR(5-氨基咪唑-4-甲酰胺核苷酸)是一种腺苷酸类似物，被肌肉细胞摄取后磷酸化形成5-甲基咪唑-4-甲酰胺-1-核糖呋喃-5-单磷酸(ZMP)，ZMP激活AMPK，促进骨骼肌的糖转运(1)。AICAR广泛用于糖摄取，糖尿病，胰岛素抵抗和运动时能量调节的测定。在ATP不足时，AICAR可以做为核苷库的一部分，快速显著增加腺苷水平(2)。

1. Sakoda, H. et al. (2002) Am. J. Physiol. Endocrinol. Metab. 282, E1239-E1244.
2. Mullane, K. et al. (1993) Trends Cardiovasc. Med. 3, 227-234.
3. Jakobsen, S. N. et al. (2001) J. Biol. Chem. 276, 46912-46916.
4. Jessen, N. et al. (2003) J. Appl. Physiol. 94, 1373-1379.
5. Giri, S. et al. (2004) J. Neurosci. 24, 479-487.

原厂资料：

Description

AICAR is supplied at 250 mM concentration. For a 0.5 mM working concentration, dilute 1:500. For a 2 mM working concentration, dilute 1:125. Treat cells with desired concentration for 5-60 minutes. Please note: Appearance of AICAR solution should be a clear to yellow/brown solution without precipitation. If precipitation is observed, warm tightly capped vial at 37C until AICAR goes into solution. Agitation of warmed solution by vortex may be necessary.

Directions for Use

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures. AICAR is supplied at 250 mM in water. Thaw completely before use. For a 0.5 mM working concentration, dilute 1:500. For a 2 mM working concentration, dilute 1:125. Treat cells with desired concentration for 5-60 minutes.

Background

AICAR (5-Aminoimidazole-4-carboxyamide ribonucleoside) is an adenosine analog taken up by muscle and phosphorylated to form 5-aminoimidazole-4-carboxamide-1--D-ribofuranosyl-5''-monophosphate (ZMP), which stimulates AMPK activity and glucose transport in skeletal muscle (1). AICAR has been used in studies measuring glucose uptake, diabetes and insulin resistance, and energy regulation during exercise. AICAR acts by entering nucleoside pools and significantly increasing levels of adenosine during periods of ATP breakdown (2).

1. Sakoda, H. et al. (2002) Am. J. Physiol. Endocrinol. Metab. 282, E1239-E1244.
2. Mullane, K. et al. (1993) Trends Cardiovasc. Med. 3, 227-234.
3. Jakobsen, S. N. et al. (2001) J. Biol. Chem. 276, 46912-46916.
4. Jessen, N. et al. (2003) J. Appl. Physiol. 94, 1373-1379.
5. Giri, S. et al. (2004) J. Neurosci. 24, 479-487.

注意事项：

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

Storage: Store solution at -20°C. Protect from light. Use
within 12 months to prevent loss of potency. Aliquot to
avoid multiple freeze/thaw cycles.