Stat Antibody Sampler Kit

• 产品编号：CST-9939S      品牌：Cell Signaling Technology       原厂货号：9939S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

试剂盒中的每一个Stat抗体只识别其目标蛋白，与是否磷酸化无关。多克隆抗体是通过合成对应的人Stat1，Stat5和Stat6肽段来免疫动物产生，抗体通过protein A和多肽亲和层析法纯化。单克隆抗体是通过合成GST-stat3（对应小鼠stat3 C-端序列肽段）融合蛋白来免疫动物产生。The Stat Antibody Sampler Kit为研究Stat1, Stat3, Stat5 and Stat6多个蛋白提供了经济的方法，试剂盒中含有四个Western免疫印迹所需的一抗和二抗。Jaks (Janus 激酶)和 Stats (转录的信号传感器和激活因子)作为受体能够与多种的配体结合，如细胞因子，激素，生长因子和神经传递素。Jaks在配体介导的受体聚集后通过自身磷酸化相关受体的酪氨酸残基， Stat分子和下游的其它信号蛋白而激活（1,2）。磷酸化Stat蛋白中的保守酪氨酸位点激活SH2介导的二聚化并迅速入核。Stat二聚体结合到IRE（干扰素响应元件）和GAS（gamma干扰素激活序列）DNA元件从转录水平上调控下游基因表达（1,2）。Stats所调控反应显著的专一性和范围一部分受控于组织特异性表达不同的细胞因子受体，Jaks 和 Stats（2,3）同时也与不同的Stat成员偶联到不同的受体上的组合有关。丝氨酸残基在C-端转录激活区域的磷酸化也调控Stat1, -2, -3, -4 and -5（1）的功能。MAPK或mTOR信号通路磷酸化Stat3的Ser727位点能优化响应生长因子和细胞因子包括IFN-gamma and CNTF的转录激活（4,5）。Jak/Stat信号通路在肿瘤的形成、肿瘤的发展、血管再生、细胞运动、免疫反应和干细胞分化中起了重要的作用（6-11）。

1. Darnell Jr., J. et al. (1994) Science 264, 1415-1421.
2. Leonard, W.J. and O'Shea, J.J. (1998) Annu. Rev. Immunol. 16, 293-322.
3. Caldenhoven, E. et al. (1996) J. Biol. Chem. 271, 13221-13227.
4. Wen, Z. et al. (1995) Cell 82, 241-250.
5. Yokogami, K. et al. (2000) Curr. Biol. 10, 47-50.
6. Lim, C.P. and Cao, X. (1999) J. Biol. Chem. 274, 31055-31061.
7. Bromberg, J. F. et al. (1999) Cell 98, 295-303.
8. Su, L. et al. (1999) J. Biol. Chem. 274, 31770-31774.
9. Dentelli, P. et al. (1999) J. Immunol. 163, 2151-2159.
10. Cattaneo, E. et al. (1999) Trends Neurosci. 22, 365-369.
11. Frank, D.A. (1999) Mol. Med. 5, 432-456.

原厂资料：

Specificity / Sensitivity

Each Stat antibody in the kit recognizes only its target protein, independent of phosphorylation state.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues of human Stat1, human Stat5 and human Stat6. Antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibody is produced by immunizing animals with GST-Stat3 fusion protein corresponding to the carboxy-terminal sequence of mouse Stat3 protein.

Description

The Stat Antibody Sampler Kit provides an economical means to examine multiple Stat proteins: Stat1, Stat3, Stat5 and Stat6. The kit contains enough primary and secondary antibodies to perform four Western blot experiments.

Background

Jaks (Janus Kinases) and Stats (Signal Transducers and Activators of Transcription) are utilized by receptors for a wide variety of ligands including cytokines, hormones, growth factors and neurotransmitters. Jaks, activated via autophosphorylation following ligand-induced receptor aggregation, phosphorylate tyrosine residues on associated receptors, Stat molecules and other downstream signaling proteins (1,2). The phosphorylation of Stat proteins at conserved tyrosine residues activates SH2-mediated dimerization followed rapidly by nuclear translocation. Stat dimers bind to IRE (interferon response element) and GAS (gamma interferon-activated sequence) DNA elements, resulting in the transcriptional regulation of downstream genes (1,2). The remarkable range and specificity of responses regulated by the Stats is determined in part by the tissue-specific expression of different cytokine receptors, Jaks and Stats (2,3), and by the combinatorial coupling of various Stat members to different receptors. Serine phosphorylation in the carboxy-terminal transcriptional activation domain has been shown to regulate the function of Stat1, -2, -3, -4 and -5 (1). Phosphorylation of Stat3 at Ser727 via MAPK or mTOR pathways is required for optimal transcriptional activation in response to growth factors and cytokines including IFN-gamma and CNTF (4,5). Jak/Stat pathways also play important roles in oncogenesis, tumor progression, angiogenesis, cell motility, immune responses and stem cell differentiation (6-11).

1. Darnell Jr., J. et al. (1994) Science 264, 1415-1421.
2. Leonard, W.J. and O'Shea, J.J. (1998) Annu. Rev. Immunol. 16, 293-322.
3. Caldenhoven, E. et al. (1996) J. Biol. Chem. 271, 13221-13227.
4. Wen, Z. et al. (1995) Cell 82, 241-250.
5. Yokogami, K. et al. (2000) Curr. Biol. 10, 47-50.
6. Lim, C.P. and Cao, X. (1999) J. Biol. Chem. 274, 31055-31061.
7. Bromberg, J. F. et al. (1999) Cell 98, 295-303.
8. Su, L. et al. (1999) J. Biol. Chem. 274, 31770-31774.
9. Dentelli, P. et al. (1999) J. Immunol. 163, 2151-2159.
10. Cattaneo, E. et al. (1999) Trends Neurosci. 22, 365-369.
11. Frank, D.A. (1999) Mol. Med. 5, 432-456.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibodies.