Src Antibody Sampler Kit

• 产品编号：CST-9935S      品牌：Cell Signaling Technology       原厂货号：9935S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

该试剂盒中的每个抗体都能检测内源性的指定位点磷酸化的Src蛋白水平。该抗体可能与相同位点磷酸化Src其他家族成员发生交叉反应。该单克隆抗体通过使用与人源Src蛋白酪氨酸（527位点）周围残基相一致的合成磷酸化或非磷酸化肽段免疫动物而获得。小鼠的单克隆抗体通过使用与人源Src蛋白酪氨酸（416位点）周围残基相一致的合成非磷酸化肽段免疫动物而获得。P-Src Y416的兔单克隆抗体通过使用与人源Src蛋白酪氨酸（416位点）周围残基相一致的合成磷酸化肽段免疫动物而获得。Src (32G6) 兔单克隆抗体通过使用与人源Src蛋白胺端周围残基相一致的合成肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。Src Antibody Sampler kit提供了一个经济的方法评价总Src蛋白水平和它的磷酸化状态。该试剂盒包含足够的一抗、二抗完成四个免疫印记实验。Src家族蛋白酪氨酸激酶包括Src, Lyn, Fyn, Yes, Lck, Blk, Hck, 对调节真核细胞的生长和分化很重要(1)。Src的活性是通过两个酪氨酸位点的磷酸化来调控的，但是这两者的调控效应相反。激酶结构域的活化环中的Tyr416位点的磷酸化，能够上调酶活性；而蛋白羧基端尾部Tyr527位点被Csk磷酸化后，则会使酶的活性降低(2)。Lyn是Src家族成员，该家族主要表达于造血细胞中(3)。Lyn参与多种细胞表面受体的信号转导，如B细胞Ag受体 (BCR)、CD40 (4)。Lck对T淋巴细胞活化和分化十分重要(5,6)。Lck的活性由蛋白激酶和磷酸酶调节。Lck蛋白羧端酪氨酸505位点的磷酸化能下调Lck的催化活性，而酪氨酸394位点的磷酸化会导致Lck活性的提高(7)。

1. Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609.
2. Hunter, T. (1987) Cell 49, 1-4.
3. Yamanashi, Y. et al. (1989) Proc. Natl. Acad. Sci. USA 86, 6538-6542.
4. Yamanashi, Y. et al. (1991) Science 251, 192-194.
5. Molina, T. J. et al. (1992) Nature 357, 161-164.
6. Straus, D. B. et al. (1992) Cell 70, 585-593.
7. Chow, L. M. et al. (1993) Nature 365, 156-160.

原厂资料：

Specificity / Sensitivity

Each antibody in the kit detects endogenous levels of Src only when in the indicated phosphorylation state at the indicated residue. The antibodies may cross-react with other Src family members when in equivalent phosphorylation states.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic phospho- or non-phosphopeptides corresponding to residues surrounding Tyr527 of human Src protein. The mouse monoclonal antibody is produced by immunizing animals with a synthetic non-phosphopeptide corresponding to residues surrounding Tyr416 of human Src protein. The rabbit monoclonal antibody for P-Src Y416 is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr416 of human Src protein. The Src (32G6) rabbit monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino-terminus of human Src. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Description

The Src Antibody Sampler kit provides an economical means of evaluating total Src protein levels and its phosphorylation status. The kit contains enough primary and secondary antibodies to perform four Western blot experiments per primary antibody.

Background

The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).Lyn is a member of the Src family that is predominantly expressed in hematopoietic cells (3). Lyn participates in signaling from multiple cell surface receptors such as the B cell Ag receptor (BCR) and CD40 (4). 
 Lck is essential for T-lymphocyte activation and differentiation (5,6). The activity of Lck is regulated by protein kinases and phosphatases. Phosphorylation of the C-terminal tyrosine 505 serves to downregulate Lck catalytic activity, while phosphorylation at tyrosine 394 leads to an increase in Lck activity (7).

1. Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609.
2. Hunter, T. (1987) Cell 49, 1-4.
3. Yamanashi, Y. et al. (1989) Proc. Natl. Acad. Sci. USA 86, 6538-6542.
4. Yamanashi, Y. et al. (1991) Science 251, 192-194.
5. Molina, T. J. et al. (1992) Nature 357, 161-164.
6. Straus, D. B. et al. (1992) Cell 70, 585-593.
7. Chow, L. M. et al. (1993) Nature 365, 156-160.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibodies.