Cleaved Caspase Antibody Sampler Kit

• 产品编号：CST-9929S      品牌：Cell Signaling Technology       原厂货号：9929S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

Cleaved Caspase-3 (Asp175) 、Cleaved Caspase-6 (Asp162)、Cleaved Caspase-7 (Asp198)、Cleaved Caspase-9 (Asp315)和Cleaved PARP (Asp214) Antibodies 抗体能够检测相应靶点的内源 Caspase 裂解的大片段。Cleaved caspase-9 Antibody 抗体能够检测37 kDa蛋白大片段+ prodomain和17 kDa大片段。Cleaved caspase-9 (Asp330) 能够检测裂解的37 kDa 大片段和17KD大片段。这些抗体不与其对应的全长蛋白发生交叉反应。该单克隆抗体是采用合成的人源caspase-3 邻近Asp175氨基酸末端序列或人源PARP Asp214处氨基酸序列相应的肽段免疫动物后制备。该多克隆抗体是用合成的人源 caspase-7、-9 或 大鼠caspase-6蛋白裂解位点处氨基酸末端残基序列相应的肽段免疫动物后制备，其是通过蛋白A和肽段亲和层析而纯化。The Cleaved Caspase Antibody Sampler Kit 通过检测它们的裂解产物，为分析caspases活化状态提供了较经济的方法。该试剂盒包含足够的一抗和二抗的量，每种抗体足够做四次免疫印迹（WB）实验。细胞凋亡是一个被调控的生理过程，导致细胞死亡。Caspases，是一个半胱氨酸蛋白酶家族，是细胞凋亡的中心调控因子。引发剂 caspases（包括8、9、10和12）被紧密耦合到促凋亡信号。一旦被激活，这些 caspases 切割并激活下游的效应 caspases（包括3、6和7），它们反过来切割细胞骨架和核蛋白质，如PARP、α-胞衬蛋白、DFF和 lamin A，并诱导细胞凋亡。从线粒体释放的细胞色素c耦合到活化的caspase-9，这是一种胱天蛋白酶引发剂（1）。促凋亡刺激物包括FasL、TNF - α、DNA损伤和内质网应激。Fas和TNFR激活 caspases 8和10（2），DNA损伤导致caspase - 9的活化，同时ER应激导致钙离子介导的caspase-12活化（3）。凋亡蛋白抑制子（IAP）家族包括XIAP和Survivin，通过结合及抑制 caspases 发挥功能（4,5）。线粒体应激时，线粒体蛋白Smac/Diablo被释放到细胞质，与半胱天冬酶竞争结合IAPs。Smac/Diablo和IAPs的相互作用解除了IAPs对半胱天冬酶的抑制效应（6）。

1. Baker, S.J. and Reddy, E.P. (1998) Oncogene 17, 3261-3270.
2. Budihardjo, I. et al. (1999) Annu. Rev. Cell Dev. Biol. 15, 269-290.
3. Nakagawa, T. et al. (2000) Nature 403, 98-103.
4. Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223.
5. Li, F. et al. (1998) Nature 396, 580-584.
6. Du, C. et al. (2000) Cell 102, 33-42.

原厂资料：

Specificity / Sensitivity

Cleaved Caspase-3 (Asp175), Cleaved Caspase-6 (Asp162), Cleaved Caspase-7 (Asp198), Cleaved Caspase-9 (Asp315), and Cleaved PARP (Asp214) Antibodies detect endogenous levels of the large cleavage fragments of their respective targets. Cleaved Caspase-9 (Asp330) Antibody detects the 37 kDa cleaved large fragment + prodomain and the 17 kDa large fragment. These antibodies will not cross-react with their respective full-length proteins.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to amino-terminal residues adjacent to Asp175 of human caspase-3 or to residues surrounding Asp214 in human PARP. Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to the amino-terminal residues adjacent to the proteolytic cleavage sites of human caspase-7, -9 or rat caspase-6. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Description

The Cleaved Caspase Antibody Sampler Kit provides an economical means to evaluate the activation status of caspases by detecting their cleaved forms. The kit contains enough primary and secondary antibodies to perform four western blot experiments with each primary antibody.

Background

Apoptosis is a regulated physiological process leading to cell death. Caspases, a family of cysteine acid proteases, are central regulators of apoptosis. Initiator caspases (including 8, 9, 10 and 12) are closely coupled to proapoptotic signals. Once activated, these caspases cleave and activate downstream effector caspases (including 3, 6 and 7), which in turn cleave cytoskeletal and nuclear proteins like PARP, α-fodrin, DFF and lamin A, and induce apoptosis. Cytochrome c released from mitochondria is coupled to the activation of caspase-9, a key initiator caspase (1). Proapoptotic stimuli include the FasL, TNF-α, DNA damage and ER stress. Fas and TNFR activate caspases 8 and 10 (2), DNA damage leads to the activation of caspase-9 and ER stress leads to the calcium-mediated activation of caspase-12 (3). The inhibitor of apoptosis protein (IAP) family includes XIAP and survivin and functions by binding and inhibiting several caspases (4,5). Smac/Diablo, a mitochondrial protein, is released into the cytosol upon mitochondrial stress and competes with caspases for binding of IAPs. The interaction of Smac/Diablo with IAPs relieves the inhibitory effects of the IAPs on caspases (6).

1. Baker, S.J. and Reddy, E.P. (1998) Oncogene 17, 3261-3270.
2. Budihardjo, I. et al. (1999) Annu. Rev. Cell Dev. Biol. 15, 269-290.
3. Nakagawa, T. et al. (2000) Nature 403, 98-103.
4. Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223.
5. Li, F. et al. (1998) Nature 396, 580-584.
6. Du, C. et al. (2000) Cell 102, 33-42.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibody.