MAPK Family Antibody Sampler Kit

• 产品编号：CST-9926S      品牌：Cell Signaling Technology       原厂货号：9926S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

MAPK Family Antibody Sampler Kit中的每个抗体只能识别它的特异性靶点，不能与其他MAP激酶发生交叉反应。p44/42 MAP Kinase Antibody通过使用与人源p44 MAP激酶羧端周围残基相一致的合成磷酸化肽段免疫动物而获得。SAPK/JNK (56G8) Rabbit mAb通过使用与人源JNK2/MBP融合蛋白免疫动物而获得。p38 MAP Kinase Antibody通过使用与人源p38 MAP激酶序列的合成肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。MAPK Family Antibody Sampler Kit提供了一个快速、经济的方法评价总p38, p44/42的蛋白水平,SAPK/JNK有丝分裂原活化的蛋白激酶水平。该试剂盒包含足够的一抗、二抗完成四个免疫印记实验。p44/42 MAPK (Erk1/2), SAPK/JNK, p38 MAPK蛋白作用于蛋白激酶级联反应中，并在调节细胞生长、分化、控制细胞应答细胞因子和应激反应中起着重要作用。p44/42 MAPK被生长和神经营养因子激活。活化的发生通过单个上游MAPK的激酶(MEK)对序列T*EY*中苏氨酸和酪氨酸残基的磷酸化（人类Erk1蛋白中Thr202位点和Tyr204位点的磷酸化）。炎性细胞因子和各种细胞应激反应也能激活SAPK/JNK和p38 MAPK蛋白。SAPK/JNK蛋白的活化通过双特异性酶SEK/MKK4对Thr183位点和Tyr185位点的磷酸化。MKK3和SEK都能磷酸化p38 MAPK蛋白 T*GY*序列的苏氨酸和酪氨酸，以激活p38 MAPK (1-5)。

1. Lewis, T. S. et al. (1998) Adv. Cancer Res. 74, 49-139.
2. Garrington, T.P. and Johnson, G.L. (1999) Curr. Opin. Cell. Biol. 11, 211-218.
3. Schaeffer, H.J. and Weber, M.J. (1999) Mol. Cell. Biol. 19, 2435-2444.
4. Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem. Sci. 23, 481-485.
5. Cobb, M.H. (1999) Prog. Biophys. Mol. Biol. 71, 479-500.

原厂资料：

Specificity / Sensitivity

Each antibody in the MAPK Family Antibody Sampler Kit recognizes only its specific target. The antibodies do not cross-react with other MAP kinases.

Source / Purification

p44/42 MAP Kinase Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the C-terminus of rat p44 MAP kinase. SAPK/JNK (56G8) Rabbit mAb is produced by immunizing animals with a human JNK2/MBP fusion protein. p38 MAP Kinase Antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human p38 MAP kinase. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Description

The MAPK Family Antibody Sampler Kit provides an economical means of evaluating total levels of p38, p44/42, and SAPK/JNK mitogen-activated protein kinases. The kit contains enough primary and secondary antibody to perform four western blot experiments.

Background

p44/42 MAPK (Erk1/2), SAPK/JNK, and p38 MAPK function in protein kinase cascades that play a critical role in the regulation of cell growth, differentiation, and control of cellular responses to cytokines and stress. p44/42 MAPK is activated by growth and neurotrophic factors. Activation occurs through phosphorylation of threonine and tyrosine residues (Thr202 and Tyr204 in human Erk1) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). SAPK/JNK and p38 MAPK are activated by inflammatory cytokines and by a wide variety of cellular stresses. Activation of SAPK/JNK occurs via phosphorylation at Thr183 and Tyr185 by the dual specificity enzyme SEK/MKK4. Both MKK3 and SEK phosphorylate p38 MAPK on tyrosine and threonine at the sequence T*GY* to activate p38 MAP kinase (1-5).

1. Lewis, T. S. et al. (1998) Adv. Cancer Res. 74, 49-139.
2. Garrington, T.P. and Johnson, G.L. (1999) Curr. Opin. Cell. Biol. 11, 211-218.
3. Schaeffer, H.J. and Weber, M.J. (1999) Mol. Cell. Biol. 19, 2435-2444.
4. Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem. Sci. 23, 481-485.
5. Cobb, M.H. (1999) Prog. Biophys. Mol. Biol. 71, 479-500.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibodies