Phospho-Syk Antibody Sampler Kit

• 产品编号：CST-9925S      品牌：Cell Signaling Technology       原厂货号：9925S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

Phospho-Syk (Ser323) 和 Phospho-Syk (Tyr525/526) 抗体可检测在Tyr323 和 Tyr 525/526位点磷酸化的人源Syk. Syk抗体和 Phospho-Zap-70 (Tyr319)/Syk (Tyr352)抗体分别检测人内源总体 Syk 和 Tyr352 位点磷酸化的Syk蛋白。每个phospho-Syk抗体只检测识别各自在特异位点磷酸化Syk蛋白. Syk的对照抗体能同时检测磷酸化和非磷酸化的Syk蛋白。Phospho-Zap-70 (Tyr319)/Syk (Tyr352) 抗体能与磷酸化形式的Zap-70交叉反应。试剂盒中的其它抗体均不与其它相关的家族中磷酸化或非磷酸化形式蛋白交叉反应。此多克隆抗体是通过合成人源性Syk Tyr323, Tyr352 or Tyr525/526周围的磷肽段免疫动物而获得。 Syk的对照抗体是通过合成未磷酸化的Syk C-末端对应的肽段来免疫兔子而获得。多克隆抗体是通过protein A和多肽亲和层析法纯化。Phospho-Syk Sampler Kit为活性形式Syk的研究提供了经济的方法，包括Syk在Tyr323, Tyr352 和 Tyr525/526位点的磷酸化。试剂盒中包含了足够做四次Western blot的一抗和二抗。Syk作为酪氨酸蛋白激酶在造血细胞的胞内信号转导中起了重要的作用(1-3)。 Syk能够与免疫受体的酪氨酸介导的活性区域(ITAMs)中的胞质部分结合(4)。它能偶联激活了的免疫受体进行下游的信号转导而引起一系列的细胞响应，如细胞增殖，分化以及吞噬(4)。有证据显示Syk在无免疫反应的细胞中也有作用，Syk在人类的乳腺癌中是个潜在的肿瘤抑制因子(5)。 Syk蛋白的SH2-激酶结合区域中Tyr323位点是个磷酸化的负调控位点 。 磷酸化的 Tyr323 为 Cbl的 TKB区域提供了结合位点(6,7)。 Syk 的酪氨酸352位点参与了与 PLC-γ1 的结合 (8)。 酪氨酸525 位点和酪氨酸526 位点位于 Syk 的激酶区域的活性环上。磷酸化人源的 Syk 的Tyr525/526位点(等同于小鼠 Syk的 Tyr519/520位点) 对 Syk 的功能有重要的影响(9)。

1. Cheng, A.M. and Chan, A.C. (1997) Curr Opin Immunol 9, 528-33.
2. Kurosaki, T. (1997) Curr Opin Immunol 9, 309-18.
3. Chu, D.H. et al. (1998) Immunol Rev 165, 167-80.
4. Turner, M. et al. (2000) Immunol Today 21, 148-54.
5. Coopman, P.J. et al. (2000) Nature 406, 742-7.
6. Deckert, M. et al. (1998) J Biol Chem 273, 8867-74.
7. Rao, N. et al. (2001) EMBO J 20, 7085-95.
8. Law, C.L. et al. (1996) Mol Cell Biol 16, 1305-15.
9. Zhang, J. et al. (2000) J Biol Chem 275, 35442-7.

原厂资料：

Specificity / Sensitivity

Phospho-Syk (Ser323) and Phospho-Syk (Tyr525/526) Antibodies detect transfected, Tyr323 and Tyr 525/526 phosphorylated human Syk. Syk Antibody and Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibodies detect endogenous levels of total human Syk and Tyr352 phosphorylated Syk, respectively. Each phospho-Syk antibody recognizes only the specific phosphorylated form of Syk. The control Syk Antibody recognizes both nonphosphorylated and phosphorylated forms of Syk. Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody cross-reacts with the phosphorylated form of Zap-70. All other antibodies in the kit do not cross-react with phosphorylated or nonphosphorylated forms of other related family members.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Tyr323, Tyr352 or Tyr525/526 of human Syk. The control Syk Antibody is produced by immunizing rabbits with a synthetic nonphosphopeptide corresponding to the carboxy terminal sequence of human Syk. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Description

Phospho-Syk Sampler Kit provides an economical means to evaluate the activation status of Syk, including the phosphorylation of Tyr323, Tyr352 and Tyr525/526. The control Syk Antibody is also included. The kit contains enough primary and secondary antibodies for four Western blot experiments.

Background

Syk is a protein tyrosine kinase that plays an important role in intracellular signal transduction in hematopoietic cells (1-3). Syk interacts with immunoreceptor tyrosine-based activation motifs (ITAMs) located in the cytoplasmic domains of immune receptors (4). It couples the activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation and phagocytosis (4). There is also evidence of a role for Syk in nonimmune cells, and Syk is a potential tumor suppressor in human breast carcinomas (5). Tyr323 is a negative regulatory phosphorylation site within the SH2-kinase linker region in Syk. Phosphorylation of Tyr323 provides a direct binding site to the TKB domain of Cbl (6,7). Tyrosine 352 of Syk is involved in the association of PLC-γ1 (8). Tyrosines 525 and 526 are located in the activation loop of the Syk kinase domain, and phosphorylation of Tyr525/526 of human Syk (equivalent to the Tyr519/520 of mouse Syk) is essential for Syk function (9).

1. Cheng, A.M. and Chan, A.C. (1997) Curr Opin Immunol 9, 528-33.
2. Kurosaki, T. (1997) Curr Opin Immunol 9, 309-18.
3. Chu, D.H. et al. (1998) Immunol Rev 165, 167-80.
4. Turner, M. et al. (2000) Immunol Today 21, 148-54.
5. Coopman, P.J. et al. (2000) Nature 406, 742-7.
6. Deckert, M. et al. (1998) J Biol Chem 273, 8867-74.
7. Rao, N. et al. (2001) EMBO J 20, 7085-95.
8. Law, C.L. et al. (1996) Mol Cell Biol 16, 1305-15.
9. Zhang, J. et al. (2000) J Biol Chem 275, 35442-7.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5),
150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at
–20°C. Do not aliquot the antibody