Phospho-Estrogen Receptor α Antibody Sampler Kit

• 产品编号：CST-9924S      品牌：Cell Signaling Technology       原厂货号：9924S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
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描述：

只有当丝氨酸（167位）磷酸化时，Phospho-Estrogen Receptor α (Ser167) (D1A3) Rabbit mAb能够检测内源性水平的雌激素受体α蛋白，并与约77 kDa的非特异性条带交叉反应。Phospho-Estrogen Receptor α (Ser104/106) Antibody能够检测内源性水平的丝氨酸（104/106位）磷酸化的雌激素受体α。Phospho-Estrogen Receptor α (Ser118) (16J4) Mouse mAb将能够检测内源性水平的丝氨酸（118位）磷酸化的雌激素受体α。Estrogen Receptor α (D8H8) Rabbit mAb能够检测内源性水平的雌激素受体α。试剂盒中的每个抗体都不与磷酸化或非磷酸化的雌激素受体β亚型或其他相关家族成员发生交叉反应。该激活状态的单克隆抗体通过用合成磷酸肽或非磷酸肽免疫动物制备，该合成磷酸肽是人雌激素受体α蛋白丝氨酸（167，118位）附近的残基和靠近羧基末端的残基。该激活状态的多克隆抗体通过用合成磷酸肽免疫动物制备，该合成磷酸肽是人雌激素受体α蛋白丝氨酸（104/106位）附近的残基。多克隆抗体由蛋白A和肽亲和层析纯化。Phospho-Estrogen Receptor α Antibody Sampler Kit提供一种经济的方法来评估雌激素受体α的激活状态，包括丝氨酸（104/106，167和118位）的磷酸化。monoclonal control ERα antibody还包括能够检测总的雌激素受体α水平。该试剂盒包含足够的一抗，可以完成4次Western blot实验。雌激素受体α(ERα)，是类固醇受体超家族的一员，含有高度保守的DNA结合域（DBD）和配体结合域（LBD）（1）。ERα通过其雌激素非依赖性和雌激素依赖性激活域（分别为AF-1和AF-2），调节转录招募共激活因子蛋白和与一般转录机制的相互作用（2）。磷酸化提供了调节ERα活性的重要机制（3,4）。 ERα有多个磷酸化位点（5）。丝氨酸（104，106，118和167位）都位于氨基末端的转录激活功能域AF-1，这些丝氨酸的磷酸化在调节ERα活性中起着重要作用。丝氨酸（118位）可能是转录调节激酶CDK7的底物（5）。丝氨酸（167位）可以通过p90RSK和Akt磷酸化（4,6）。 丝氨酸（167位）的磷酸化可能会使乳腺癌患者对他莫昔芬耐药（4）。

1. Mangelsdorf, D.J. et al. (1995) Cell 83, 835-9.
2. Glass, C.K. and Rosenfeld, M.G. (2000) Genes Dev 14, 121-41.
3. Chen, D. et al. (1999) Mol Cell Biol 19, 1002-15.
4. Campbell, R.A. et al. (2001) J Biol Chem 276, 9817-24.
5. Chen, D. et al. (2000) Mol Cell 6, 127-37.
6. Joel, P.B. et al. (1998) Mol Cell Biol 18, 1978-84.

原厂资料：

Specificity / Sensitivity

Phospho-Estrogen Receptor α (Ser167) (D1A3) Rabbit mAb detects endogeneous levels of ERα protein only when phosphorylated at Ser167, and also cross reacts with a nonspecific band around 77 kDa. Phospho-Estrogen Receptor α (Ser104/106) Antibody detects endogenous levels of Ser104/106 phosphorylated ERα. Phospho-Estrogen Receptor α (Ser118) (16J4) Mouse mAb will detect endogenous levels of Ser118 phosphorylated ERα. Estrogen Receptor α (D8H8) Rabbit mAb detects endogenous levels of ERα. Each antibody in the kit does not cross-react with phosphorylated or nonphosphorylated ER isoforms β or other related family members.

Source / Purification

Activation state monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides or non-phosphopeptide corresponding to residues surrounding Ser167, Ser118, and the carboxy terminus of human estrogen receptor α protein. Activation state polyclonal antibody is produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser104/106 of human ERα. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Description

The Phospho-Estrogen Receptor α Antibody Sampler Kit provides an economical means to evaluate the activation status of ERα, including phosphorylation of Ser104/106, Ser167, and Ser118. The monoclonal control ERα antibody is also included to detect total Estrogen Receptor α levels. The kit contains enough primary antibody to perform four Western blot experiments.

Background

Estrogen receptor α (ERα), a member of the steroid receptor superfamily, contains highly conserved DNA binding and ligand binding domains (1). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ERα regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery (2).Phosphorylation at multiple sites provides an important mechanism to regulate ERα activity (3-5). Ser104, 106, 118, and 167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serine residues plays an important role in regulating ERα activity. Ser118 may be the substrate of the transcription regulatory kinase CDK7 (5). Ser167 may be phosphorylated by p90RSK and Akt (4,6). According to the research literature, phosphorylation at Ser167 may confer tamoxifen resistance in breast cancer patients (4).

1. Mangelsdorf, D.J. et al. (1995) Cell 83, 835-9.
2. Glass, C.K. and Rosenfeld, M.G. (2000) Genes Dev 14, 121-41.
3. Chen, D. et al. (1999) Mol Cell Biol 19, 1002-15.
4. Campbell, R.A. et al. (2001) J Biol Chem 276, 9817-24.
5. Chen, D. et al. (2000) Mol Cell 6, 127-37.
6. Joel, P.B. et al. (1998) Mol Cell Biol 18, 1978-84.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibodies