Rapamycin

• 产品编号：CST-9904S      品牌：CST       原厂货号：9904S
• 产品分类：细胞,细胞培养与转染试剂 > 抗生素和抗真菌剂 > 大环内酯类抗生素
• 应用分类：

描述：

Rapamycin（雷帕霉素）溶于DMSO和甲醇。用于培养的细胞实验时，CST推荐刺激细胞前先用10 nM的rapamycin处理一个小时。Rapamycin（雷帕霉素）是冻干粉形式提供的。配置100 uM储液时，将10 nmol（9.1 ug）的rapamycin溶于100 ul的甲醇或DMSO中。Rapamycin(雷帕霉素)是一种具有抗真菌和免疫抑制活性的细菌大环内酯（1）。Rapamycin与亲免素FKBP12形成复合物，从而抑制FRAP/ mTOR的活性（酵母中的TOR）（2,3）。雷帕霉素处理细胞后，导致p70 S6激酶去磷酸化并失活。雷帕霉素也导致4E-BP1/PHAS1去磷酸化，从而促进其与eIF4E结合并抑制其活性（4,5）。这个特性已被证明是雷帕霉素阻止蛋白质合成，及使细胞周期停滞在G1期的基础（6,7）。然而，也有人认为雷帕霉素抑制G1 / S期转换可能是由于其对cyclin D1 mRNA和蛋白质稳定性影响的结果（8）。Rapamycin（雷帕霉素）溶于DMSO和甲醇。用于培养的细胞实验时，CST推荐您刺激细胞前先用10 nmol的rapamycin处理一个小时。

1. Dumont, F. J. et al. (1990) J. Immunol. 144, 251-258.
2. Brown, E. J. et al. (1994) Nature 369, 756-758.
3. Kunz, J. et al. (1993) Cell 73, 585-596.
4. Jefferies, H. B. et al. (1997) EMBO J. 15, 3693-3704.
5. Beretta, L. et al. (1996) EMBO J. 15, 658-664.
6. Thomas, G. and Hall, M.N. (1997) Curr. Opin. Cell. Biol. 9, 782-787.
7. Dennis, P. B. et al. (1999) Curr. Opin. Genet. & Develop. 9, 49-54.
8. Hashemolhosseini, S. et al. (1998) J. Biol. Chem. 273, 14424-14429.

原厂资料：

Description

Rapamycin is soluble in DMSO and methanol. For experiments with cultured cells, CST recommends pretreating with 10 nM of this inhibitor for one hour prior to stimulation.

Directions for Use

Rapamycin is supplied as a lyophilized powder. For a 100 µM stock, resuspend 10 nmol (9.1 µg) in 100 µl methanol or DMSO.

Background

Rapamycin is a bacterial macrolide with antifungal and immunosuppressant activities (1). Rapamycin forms a complex with the immunophilin FKBP12 which then inhibits the activity of FRAP/ mTOR (TOR in yeast) (2,3). Rapamycin treatment of cells leads to the dephosphorylation and inactivation of p70 S6 kinase. Rapamycin also leads to the dephosphorylation of 4E-BP1/PHAS1, thereby promoting its binding to and inactivation of eIF4E (4,5). This activity has been shown to be the basis for Rapamycin's ability to block protein synthesis and to arrest cell cycle progression in the G1-phase (6,7). However, it has been suggested that Rapamycin's inhibition of the G1/S transition may be the consequence of its effect on cyclin D1 mRNA and protein stability (8).Rapamycin is soluble in DMSO and methanol. For experiments with cultured cells, CST recommends pretreating with 10 nmol of this inhibitor for one hour prior to stimulation.

1. Dumont, F. J. et al. (1990) J. Immunol. 144, 251-258.
2. Brown, E. J. et al. (1994) Nature 369, 756-758.
3. Kunz, J. et al. (1993) Cell 73, 585-596.
4. Jefferies, H. B. et al. (1997) EMBO J. 15, 3693-3704.
5. Beretta, L. et al. (1996) EMBO J. 15, 658-664.
6. Thomas, G. and Hall, M.N. (1997) Curr. Opin. Cell. Biol. 9, 782-787.
7. Dennis, P. B. et al. (1999) Curr. Opin. Genet. & Develop. 9, 49-54.
8. Hashemolhosseini, S. et al. (1998) J. Biol. Chem. 273, 14424-14429.

注意事项：

Storage: Store lyophilized or in solution at -20ºC, desiccated.
In lyophilized form, the chemical is stable for 24
months. Once in solution, use within 3 months to prevent
loss of potency. Aliquot to avoid multiple freeze/thaw cycles.