Roscovitine

• 产品编号：CST-9885S      品牌：CST       原厂货号：9885S
• 产品分类：生化试剂 > 生物试剂 > 生物活性小分子 > 抑制剂
• 应用分类：

描述：

Roscovitine [(R) stereoisomer] 为1mg粉末。-20ºC或更低温度保存。使用前用143 μl DMSO或MeOH稀释溶解至20mM贮存液。推荐工作浓度为组织培养液中20 μM 。处理细胞4-24小时，贮存于-20ºC。核抑制剂是一种能透过细胞的周期蛋白依赖性激酶CDK1 (cdc2)，CDK2和CDK5的选择性抑制剂(1)。作为一种嘌呤类似物，该药物竞争性结合催化裂口的ATP位点。使用roscovitine处理培养的细胞能够引起细胞周期捕获或者凋亡(1-4)。该药对于cdc2活性的体外IC50是0.65 μM (1)。

1. Meijer, L. et al. (1997) Eur J Biochem 243, 527-36.
2. Whittaker, S.R. et al. (2007) Cell Cycle 6, 3114-31.
3. Dey, A. et al. (2008) Cell Death Differ 15, 263-73.
4. Wojciechowski, J. et al. (2003) Int J Cancer 106, 486-95.

原厂资料：

Directions for Use

Roscovitine [(R) stereoisomer] is supplied as a 1 mg powder. Store at or below -20ºC. Before use, dissolve powder in 143 μl DMSO or MeOH to make a 20 mM stock solution. The suggested working concentration is 20 μM in tissue culture medium. Treat cells for 4-24 hours. Store solution at -20ºC.

Background

Roscovitine is a cell permeable reversible selective inhibitor of cyclin-dependent kinases CDK1 (cdc2), CDK2 and CDK5 (1). A purine analog, this drug competes for the binding site of ATP in the catalytic cleft. Treatment of cultured cells with roscovitine can cause cell cycle arrest or apoptosis (1-4). The IC50 for cdc2 activity is 0.65 μM in vitro (1). 

1. Meijer, L. et al. (1997) Eur J Biochem 243, 527-36.
2. Whittaker, S.R. et al. (2007) Cell Cycle 6, 3114-31.
3. Dey, A. et al. (2008) Cell Death Differ 15, 263-73.
4. Wojciechowski, J. et al. (2003) Int J Cancer 106, 486-95.

注意事项：

Storage: Store lyophilized or in solution at -20°C, desiccated.
In lyophilized form, the chemical is stable for 24 months. Once in
solution, use within 3 months to prevent loss of potency. Aliquot
to avoid multiple freeze/thaw cycles.