DDX5 (D15E10) XP? Rabbit mAb

• 产品编号：CST-9877S      品牌：CST       原厂货号：9877S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

DDX5 (D15E10) XP® Rabbit mAb兔单抗检测内源性DDX5总蛋白。通过人工合成人源DDX5蛋白相应的多肽片段去免疫动物从而制备出单克隆抗体。DDX5 (DEAD盒子多肽5)又称为p68，其起初被鉴定为和翻译起始因子eIF-4A蛋白类似的一个68 kDa大小的核蛋白(1)。DDX5是推测的DEAD盒子RNA解螺酶家族成员，通常其含有保守的DEAD (Asp-Glu-Ala-Asp)基序，而该基序的主要功能是调节RNA的二级结构。DDX5蛋白具有ATP-依赖的RNA解旋酶活性，以及该蛋白作为调节miRNA和rRNA加工的的DROSHA复合物重要组分(3,4)。DDX也可能调节mRNA剪接(5)，并且证实和HDAC1相互作用，而HDAC1是能够调节启动子特异性转录(6)。DDX5能够和多种不同的蛋白质相互作用，包括Runx2, p53, Smad3, CBP和p300 (7-10)，研究表明DDX5蛋白在生物发育进程中的多个维度起到重要作用。尤其是DDX5蛋白可能涉及到生长因子诱导上皮-间质转化（EMT）。在PDGF刺激下DDX5在Tyr593位点磷酸化，使Axin与β-catenin解离； 这阻断了GSK-3β对β-catenin磷酸化，从而促进Wnt蛋白依赖的β-catenin核迁移(11)以及促使c-Myc, cyclin D1和Snai1基因转录(12,13

1. Ford, M.J. et al. (1988) Nature 332, 736-8.
2. Hirling, H. et al. (1989) Nature 339, 562-4.
3. Fukuda, T. et al. (2007) Nat Cell Biol 9, 604-11.
4. Davis, B.N. et al. (2008) Nature 454, 56-61.
5. Camats, M. et al. (2008) PLoS ONE 3, e2926.
6. Wilson, B.J. et al. (2004) BMC Mol Biol 5, 11.
7. Jensen, E.D. et al. (2008) J Cell Biochem 103, 1438-51.
8. Bates, G.J. et al. (2005) EMBO J 24, 543-53.
9. Warner, D.R. et al. (2004) Biochem Biophys Res Commun 324, 70-6.
10. Rossow, K.L. and Janknecht, R. (2003) Oncogene 22, 151-6.
11. Yang, L. et al. (2006) Cell 127, 139-55.
12. Yang, L. et al. (2007) J Biol Chem 282, 16811-9.
13. Carter, C.L. et al. (2010) Oncogene 29, 5427-36.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Bovine, Horse

Specificity / Sensitivity

DDX5 (D15E10) XP® Rabbit mAb recognizes endogenous levels of total DDX5 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser498 of human DDX5 protein.

Background

DDX5 (DEAD box polypeptide 5), also known as p68, was first identified as a 68 kDa nuclear protein with similarity to translation initiation factor eIF-4A (1). DDX5 is a member of the DEAD box family of putative RNA helicases, defined by the presence of a conserved DEAD (Asp-Glu-Ala-Asp) motif that appears to function primarily in the regulation of RNA secondary structure. DDX5 exhibits ATP-dependent RNA helicase activity (2) and has been identified as a critical subunit of the DROSHA complex that regulates miRNA and rRNA processing (3,4). DDX may also regulate mRNA splicing (5) and has been shown to interact with HDAC1, where it can regulate promoter-specific transcription (6). DDX5 interacts with a diverse group of proteins, including Runx2, p53, Smad3, CBP, and p300 (7-10), suggesting an important role for DDX5 in a multitude of developmental processes. Notably, DDX5 may be involved in growth factor-induced epithelial mesechymal transition (EMT). Phosphorylation of DDX5 at Tyr593 following PDGF stimulation was shown to displace Axin from β-catenin; this prevented phosphorylation of β-catenin by GSK-3β, leading to Wnt-independent nuclear translocation of β-catenin (11) and increased transcription of c-Myc, cyclin D1, and Snai1 (12,13).

1. Ford, M.J. et al. (1988) Nature 332, 736-8.
2. Hirling, H. et al. (1989) Nature 339, 562-4.
3. Fukuda, T. et al. (2007) Nat Cell Biol 9, 604-11.
4. Davis, B.N. et al. (2008) Nature 454, 56-61.
5. Camats, M. et al. (2008) PLoS ONE 3, e2926.
6. Wilson, B.J. et al. (2004) BMC Mol Biol 5, 11.
7. Jensen, E.D. et al. (2008) J Cell Biochem 103, 1438-51.
8. Bates, G.J. et al. (2005) EMBO J 24, 543-53.
9. Warner, D.R. et al. (2004) Biochem Biophys Res Commun 324, 70-6.
10. Rossow, K.L. and Janknecht, R. (2003) Oncogene 22, 151-6.
11. Yang, L. et al. (2006) Cell 127, 139-55.
12. Yang, L. et al. (2007) J Biol Chem 282, 16811-9.
13. Carter, C.L. et al. (2010) Oncogene 29, 5427-36.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody