O-GlcNAc (CTD110.6) Mouse mAb

• 产品编号：CST-9875S      品牌：CST       原厂货号：9875S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

O-GlcNAc (CTD110.6)鼠单抗特异性的识别内源性的蛋白质丝氨酸和苏氨酸上β-O-糖苷键连接的O-GlcNAc。该单克隆抗体用包含serine-O-linked N-acetylglucosamine(O-GlcNAc)的肽段免疫动物制成。O-GlcNAcylatio是一个翻译后修饰,β-D-N-acetylglucosamine (GlcNAc)被共价连接到细胞质和细胞核蛋白的丝氨酸或苏氨酸残基（1,2）。这种修饰对很多细胞过程都很重要，包括代谢、细胞生长和形态、细胞凋亡和转录（2,3），并在癌症中起关键作用（1）。

1. Comer, F.I. et al. (2001) Anal Biochem 293, 169-77.
2. Slawson, C. and Hart, G.W. (2011) Nat Rev Cancer 11, 678-84.
3. Capotosti, F. et al. (2011) Cell 144, 376-88.

原厂资料：

Specificity / Sensitivity

O-GlcNAc (CTD110.6) Mouse mAb specifically recognizes endogenous levels of O-GlcNAc on proteins in β-O-glycosidic linkage to both serine and threonine.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a peptide containing serine-O-linked N-acetylglucosamine (O-GlcNAc).

Background

O-GlcNAcylation is a post-translational modification where β-D-N-acetylglucosamine (GlcNAc) is covalently linked to cytoplasmic and nuclear proteins at serine or threonine residues (1,2). This modification is important in many cellular processes including metabolism, cell growth and morphogenesis, apoptosis and transcription (2,3) and has been implicated to play a critical role in cancer (1).

1. Comer, F.I. et al. (2001) Anal Biochem 293, 169-77.
2. Slawson, C. and Hart, G.W. (2011) Nat Rev Cancer 11, 678-84.
3. Capotosti, F. et al. (2011) Cell 144, 376-88.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.